从赤魟组织中分离到的福安肽的抗血管生成作用

Antiangiogenesis of Fuantai isolated from Dasyatis akajei

目的研究从赤魟组织中分离到的福安肽(FAT)对鸡胚绒毛尿囊膜(CAM)血管生成、人低分化鼻咽癌细胞(CNE-2Z)诱导的CAM血管生成和小鼠肝癌组织血管生成的影响。方法用CAM法检测FAT对CAM血管生成和CNE-2Z细胞诱导的CAM血管生成的影响;用免疫组化法检查FAT对小鼠肝癌组织内微血管密度(MVD)的影响。结果FAT显著抑制CAM血管生成,当其用量为30,60,120μg/(胚.d)×3时,其抑制率分别为30.8%,50.1%,64.0%;CNE-2Z细胞明显诱导CAM血管生成,当CNE-2Z细胞的接种量为1.9×106细胞/胚时,其血管生成诱导率为30.1%;FAT明显抑制CNE-2Z细胞诱导的CAM血管生成,当其用量为30,60,120μg/(胚.d)×4时,其抑制率分别为35.8%,43.1%,51.3%。FAT明显降低小鼠肝癌组织的MVD,FAT平均微血管数为(8.2±1.3)与对照组的(21.3±2.8)相比,差异显著(P<0.05)。结论FAT有显著的抗血管生成作用,这可能是其抑制小鼠肿瘤生长的重要原因。

Purpose To investigate a possible antiangiogenesis of Fuantai（FAT ）isolated from the soft tissues of Dasyatis akajei. Methods Quantitation of angiogenesis and antiangiogenesis of FAT was performed in the chick embryochorioallantoic membrane （CAM）, and transplantable mouse H22 model was applied to investigate the effect of FAT on intratumoral microvessel density （MVD）. Results FAT showed obvious inhibitory effect on angiogenesis in the CAM. When administered 30,60,120 μg/（embryo·d）for 3 days, FAT caused 30.8%, 50.1% and 64.0% inhibititory rates on angiogenesis in CAM, respectively. CNE-2Z cells significantly induced angiogenesis in CAM, and FAT markedly inhibited it. When seeded with 1.9×10^6 cells/embryo, CNE-2Z cells caused 30.1% induction rate of angiogenesis, when administered 30, 60, 120 μg/（embryo·d）for 4 days, FAT caused 35.8%, 43.1% and 53.1% inhibititory rates on CNE-2Z cell-induced angiogenesis in CAM, respectively. FAT obviously decreased intratumoral MVD in H22 （ P 〈 0.05）. Conclusion FAT shows significant inhibitory effects on angiogenesis and tumor-induced angiogenesis in CAM and H22 ,and this may be responsible for its antitumor growth.