BAEE法测定重组水蛭素的生物活性

Determination of the biological activity in recombinat Hirudin by BAEE

目的建立重组水蛭素的生物活性测定方法。方法用BAEE（N-苯甲酰-L-精氨酸乙酯）作底物，通过测定反应体系中未被水蛭素抑制的凝血酶的量，来间接测定水蛭素抗凝血酶的活力。结果重组水蛭素的生物活性测定曲线在3．2～12．8ATU之间线性关系良好，回归方程为Y=-0．0348X＋0．7，r=0．9993。结论BAEE法经济、便捷、准确可靠、重现性好，适宜作为该品种的生物活性测定方法。

Purpose To develop a method for the determination of recombinat Hirudin （rH）. Methods To determine the biological activity of rH by the thrombin which was not inhibited. Results The calibration curve was linear in the range of 3.2 - 12.8 ATU.The regression equation of rH was Y= - 0.069 6 X ＋ 0.7, r = 0. 999 3. Conclusion The BAEE method was accurate and reliable for the determination of rH.