福建黄兔的遗传多态性及分类

Genetic Diversity and Classification of Fujian Yellow Rabbit

用8个微卫星标记对福建黄兔的等位基因频率、多态信息含量、杂合度和有效等位基因数等指标进行统计分析,并在此基础上对其进行聚类分析和分类研究。结果表明:(1)8个微卫星标记在所检测的福建黄兔群体中都表现出较丰富的多态性,在6个群体微卫星标记的平均多态信息含量(PIC)为0.6622(0.5723～0.7222),各群体的PIC差异不显著;全部群体平均杂合度为0.6857(0.5904～0.7267)。这显示黄兔群体的多态信息含量、杂合度等指标有较好的一致性,说明福建黄兔品种内在微卫星位点上均具有丰富的遗传多样性。(2)根据奈氏标准遗传距离,并进行UPGMA聚类分析,结果显示6个群体间的遗传变异不大。福建黄兔地方品种在微卫星位点上具有丰富的遗传多样性;从遗传距离及聚类分析结果表明这些群体黄兔属于同一个地方品种。

The genetic diversity of eight microsatellite sites was studied by PCR,and the allele frequency,polymorphism information content（PIC）,heterozygosity（H） and effective number of alleles（Ne） in different rabbit populations were analyzed with statistics method.At the same time,the cluster of these rabbits has also been studied.The results were showed as follow that（1） The eight microsatellite sites were high polymorphism in six rabbit populations.The average PIC in each site was 0.6622（0.5723-0.7222）.The difference of average PIC among these populations was not significant.The average heterozygosity in all populations was 0.6857（0.5904-0.7267）.These results indicated that there was a good compatibility between PIC and H in different six rabbit populations.It also showed that there was rich in genetic polymorphism of microsatellite sites within Fujian yellow rabbit.（2） According to the Neis standard genetic distance,the UPGMA dendrogram indicated that there were little genetic difference among six populations.There was rich in genetic polymorphism of microsatellite sites within Fujian yellow rabbit.This cluster result showed that these populations clustered into the same breed.