摘要
为有效利用引自国际玉米小麦改良中心的175份六倍体小黑麦材料,采用SDS-PAGE电泳方法对其高分子量麦谷蛋白亚基(HMW-GS)组成进行了分析。结果表明,这些材料的HMW-GS类型丰富,在Glu-A1,Glu-B1和Glu-Rx三个位点上分别检测到3种、4种和7种不同的亚基类型;在Glu-A1位点Null亚基出现的频率最高,为49.14%,其次为1和2*频率分别为30.29%和20.57%;在Glu-B1位点13+16亚基出现的频率最高,为45.71%;黑麦染色体编码的亚基主要是21*和22*。共检测到27种HMW-GS组合类型,其中(N,21*,13+16)、(N,21*,14+15,22*)、(1,21*,13+16,22*)、(N,21*,7+8)和(1,21*,13+16)出现的频率较高,分别为10.29%、9.14%、8.57%、6.29%和5.71%。
The composition of high molecular weight glutenin subunits（HMW-GS） in 175 hexaploid Triticale from CIMMYT were analyzed using SDS-PAGE method in this experiment.The result showed that the HMW-GS composition of 175 lines were extremely abundant,3 ,4 and 7 different HMW-GS types were found in the loci of Glu-A1,Glu-B1 and Glu-Rx respectively.The frequency of subunit Null was the highest（49.14%） at Glu-A1 locus,and the second are 1（30.29%） and 2^＊ （20.57%）.（13＋16）was the highest（45.71%） at Glu-B1.The subunits coded by rye chromosome were mainly 21^＊ and 22^＊.27 HMW-GS composition types were found ,and among them the frequency of（N,21^＊,13＋16,）（10.29%）、（N,21^＊,14＋15,.22^＊）（9.14%）、（1,21^＊,13＋16,22^＊）（8.57%）、（N,21^＊,7＋8）（6.29%）和（1,21^＊,13＋16）（5.71%）was higher.
出处
《中国农学通报》
CSCD
2008年第8期74-79,共6页
Chinese Agricultural Science Bulletin
基金
农业部农业结构调整重大技术研究专项"超高产
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