摘要
以籼稻品种93-11为轮回亲本,与粳稻品种日本晴杂交并回交的高世代分离群体为研究材料,选用104个多态性的SSR标记对水稻的落粒性基因进行定位。结果表明,在BC4F2群体中,6个标记的基因型来自于日本晴;在BC4F3定位群体中,难落粒植株数与易落粒植株数的分离比例为3:1,落粒性受1对显性基因控制,命名为SH1;分子标记与落粒性共分离分析将SH1定位在SSR标记RM5389和RM1068、RM1387之间,与3个标记的遗传距离分别为0.7cM、5.5cM和13.1cM,此结果为该基因的分子标记辅助选择奠定了基础。
In the paper,with an indica variety 93-11 as recipient and a japonica variety Nipponbare as donor,high generation backcross segregation populations were constructed.Using 104 SSR markers which display polymorphism bands between 93-11 and Nipponbare,the genetic background of BC4F2 and seed shattering of BC4F3 mapping population were analyzed.The results showed that in BC4F2 population,the genetic background analysis detected 6 markers,which distributed on rice chromosomal 1,2 and chromosomal 5 derived from donar Nipponbare;In BC4F3 mapping population,non-shattering plants and easy shattering plants segregated in a ratio of 169:52,fitting the expected ratio of 3:1,suggesting that the seed shattering in the population was controlled by a dominant gene,named SH1;Linkage analyses suggested that the gene SH1 was linked with SSR marker RM5389,RM1068 and RM1387,the genetic distance between these markers and SH1 were 0.7cM,5.5cM and 13.1cM,respectively.The mapping of the gene SH1 would be useful for its molecular marker-assisted selection.
出处
《中国农学通报》
CSCD
2008年第8期84-87,共4页
Chinese Agricultural Science Bulletin
基金
国家高技术研究发展计划(863计划)项目"植物分子与细胞高效育种技术与品种创制"(2006AA100101)
国家科技支撑计划项目"长江中下游粳稻育种技术研究及新品种选育"(2006BAD01A01-5)
江苏省农业科技自主创新基金"优质超高产水稻品种选育
配套栽培技术示范与推广"(CX[07]603)。
关键词
水稻
SSR标记
落粒性基因
分子标记辅助选择
rice,SSR marker,seed shattering gene,molecular marker-assisted selection