一个水稻落粒性基因SH1的SSR标记定位

Mapping of SH1,A Dominant Gene Controlling Seed Shattering Using SSR Markers in Rice(Oryza sativa L.)

以籼稻品种93-11为轮回亲本,与粳稻品种日本晴杂交并回交的高世代分离群体为研究材料,选用104个多态性的SSR标记对水稻的落粒性基因进行定位。结果表明,在BC4F2群体中,6个标记的基因型来自于日本晴;在BC4F3定位群体中,难落粒植株数与易落粒植株数的分离比例为3:1,落粒性受1对显性基因控制,命名为SH1;分子标记与落粒性共分离分析将SH1定位在SSR标记RM5389和RM1068、RM1387之间,与3个标记的遗传距离分别为0.7cM、5.5cM和13.1cM,此结果为该基因的分子标记辅助选择奠定了基础。

In the paper,with an indica variety 93-11 as recipient and a japonica variety Nipponbare as donor,high generation backcross segregation populations were constructed.Using 104 SSR markers which display polymorphism bands between 93-11 and Nipponbare,the genetic background of BC4F2 and seed shattering of BC4F3 mapping population were analyzed.The results showed that in BC4F2 population,the genetic background analysis detected 6 markers,which distributed on rice chromosomal 1,2 and chromosomal 5 derived from donar Nipponbare;In BC4F3 mapping population,non-shattering plants and easy shattering plants segregated in a ratio of 169：52,fitting the expected ratio of 3：1,suggesting that the seed shattering in the population was controlled by a dominant gene,named SH1;Linkage analyses suggested that the gene SH1 was linked with SSR marker RM5389,RM1068 and RM1387,the genetic distance between these markers and SH1 were 0.7cM,5.5cM and 13.1cM,respectively.The mapping of the gene SH1 would be useful for its molecular marker-assisted selection.