陆地棉分子遗传图谱的构建

Construction of the Molecular Linkage Map with SSR Markers in Upland cotton

利用SSR引物对具有抗黄萎病性状的F2群体进行分子遗传图谱构建研究。结果表明:2000对SSR引物在对新陆中10号和新陆早7号的多态性筛选中,共筛选到89个稳定的SSR多态性位点,其中共显性标记为74个,显性标记15个;利用Mapmaker/EXP(version 3.0b)对89个标记构建了连锁群,其中65个标记位点被分配到19个不同的连锁群上,24个标记位点没有分配到连锁群上;19个连锁群总的长度962.2cM,覆盖率为17.49%。

A mapping population consisting of F2 individuals derived from resiting Verticillium wilt was used in this study.The 89 SSR Polymorphic Loci was filtrated in 2000 SSR Primers with Xinluzhong 10 and Xinluzao 7,74 codominant markers and 15 dominant markers were checked.The linkage groups were constructed in 89 molecular markers with Mapmaker/EXP（version 3.0b）,and 65 marker loci were distributed on 19 different linkage groups,24 marker loci were not distributed on linkage groups.The total length of 19 linkage groups was 962.2cm,and the rate of covering was 17.49%.