血清CA19-9、CEA、TGF-β1及VEGF检测在胃癌患者诊治中的意义

Analysis of CA19-9,CEA,TGF-β1 and VEGF in Serum of Patients with Gastric Carcinoma and Its Significance

【目的】探讨血清CA19-9、CEA、TGF-β1及VEGF检测在胃癌患者诊治中的意义。【方法】采用酶联免疫吸附法(ABC法)和全自动化学发光免疫法测定65例胃癌组织中CA19-9、CEA、TGF-β1及VEGF表达情况,另以10位正常体检者为对照。【结果】65例患者中,CA19-9、CEA及VEGF的表达与肿瘤的临床分期、有无淋巴转移、肿瘤直径及浸润深度明显正相关,而与性别、年龄无明显相关性。TGF-β1的表达与肿瘤的淋巴转移及浸润深度明显正相关,与性别、年龄和肿瘤直径无明显相关;术后这些指标值较术前明显降低。4项检测中,除VEGF和TGF-β1的表达具有正相关(r=0.36)外,其余指标相互之间的表达无明显相关性,而联合检测以其中一项异常为阳性,其阳性率可达84.6%。【结论】CA19-9、CEA、TGF-β1及VEGF与肿瘤的临床分期、有无淋巴转移及浸润深度明显正相关,VEGF和TGF-β1的表达具有正相关性;联合检测这4个项目可提高胃癌阳性检出率,检测这些指标不仅可以判断预后,而且可以指导治疗。

[Objective] To explore the significance of CA19-9, CEA, TGF-β1 and VEGF in serum of patients with gastric carcinoma. [Methods] Sixty five patients with gastric carcinoma and 10 healthy controls were enrolled in this study. CA19-9, CEA, TGF-β1 and VEGF in serum were detected by automatic chemoluminescence immunifaction and ELISA. [Results]In 65 cases, the expressions of CA19-9, CEA and VEGF were positively related to the tumor＇s clinical staging, lymphatic metastasis, the size of tumor and depth of infiltration, but not related to the gender and age. The expression of TGF-β1 was positively related to the tumor ＇s clinical staging, lymphatic metastasis and depth of infiltration, but was not related to the gender and age and size of tumor. All these four indexes had significantly decreased after radical correction. There were no relations among these four indexes, except the expression of TGF-β1 had positive correlation with VEGF. While combined detection, if one of two indexes abnormal was confined as positive, the positive rate could reach 84. 6 %. [Conclusion] CA19-9, CEA, TGF-β1 and VEGF may be related to the tumor＇s clinical staging, lymphatic metastasis and depth of infiltration, and the expression of TGF-β1 may have positive correlation to VEGF. Combination detection of these four indexes could increase the positive rate of gastric carcinoma. The detection of these indexes can not only judge prognosis, but guide the treatment.