黄连解毒汤中栀子苷在大鼠体内的药动学研究

Pharmacokinetic study of jasminoidin in Huanglianjiedu decoction in rats

[目的]探讨黄连解毒汤中栀子苷在大鼠体内的药动学。[方法]大鼠灌胃给药,取血经处理后采用高效液相色谱(HPLC)法测定栀子苷含量。[结果]大鼠灌胃黄连解毒汤后,体内栀子苷的药-时曲线符合二室开放模型,药动学参数为:分布速率常数(α)=(0.03±0.01)/min,消除速率常数(β)=(0.0006±0.0002)/min,二室模型分布相的半衰期(t1/2α)=(79.02±22.38)min,二室模型消除相的半衰期(t1/2β)=(13031.81±8817.76)min,达峰时间(T(peak))=(99.46±28.73)min,血药峰浓度(Cmax)=(5.02±1.52)mg/L,血药浓度-时间曲线下面积(AUC)=(69334.66±15565.92)mg/(L·min)。[结论]明确了黄连解毒汤中栀子苷在大鼠体内的药动学过程,为黄连解毒汤的临床应用提供可参考的依据。

[Objective] To study the pharmacokinetics of Jasminoidin in Huanglianjiedu Decoction in rats. [Methods] HPLC detection was used to determine the Jasminoidin levels in biological samples. [Results] After giving Jasminoidin orally in rats, the plasma concentration-time course fitted well to two-compartment model and with following pharmacolinetic parameters： α=（0.03±0.01 ）/min, β=（0.000 6±0.000 2）/min,t1/2α=（79.02±22.38） min,t1/2β=（13 031.81±8 817.76） min,T（peak）=（99.46±28.73） min,Cmax=（5.02±1.52） mg/L, AUC=（69 334.66±15 565.92） mg/（L·min）. [Conclusion] The pharmacokinetic characteristics of Jasminoidin in Huanglianjiedu Decoction in rats was identified, providing evidence for clinical medication of Huanglianjiedu decoction.