大孔吸附树脂分离纯化中药仙鹤复方总黄酮和总萜类的研究

Separation and purification of total flavones and total triterpenoids in Xianhe compound prescription with macroreticular resin

[目的]研究大孔吸附树脂分离纯化中药仙鹤复方总黄酮、总萜类的工艺条件及参数。[方法]采用静态吸附实验和动态吸附实验等考察了7种大孔树脂对中药仙鹤复方总黄酮和总萜类的分离纯化效果及影响因素。[结果]SP825型树脂具有最佳的吸附及洗脱效果。以50%乙醇为洗脱剂,总黄酮主要集中在正丁醇萃取层,而总萜类主要集中在乙酸乙酯萃取层,总黄酮收率为54.90%,含量为18.20%,总萜类收率为46.15%,含量为71.86%。[结论]SP825型树脂综合性能较好,适于中药仙鹤复方总黄酮、总萜类的分离纯化。

[Objective] To investigate the technological parameters of the purification process of total flavones and total triterpenoids from Xianhe compound prescription with macroreticular resins. [Methods] The static capacity absorption and static elution ratio were observed, the separation effect and influence factors of seven types macroreticular resins was evaluated. [Results] SP825 type macroreticular resin owned optimum absorption and eluation parameters. The total flavonids could be eluted by 50% ethanol from extract part of n-Butanol, as well as the total triterpenoids could be eluted from extract part of Ethyl acetate. The recovery of total flavones was 54.90%, product purity was 18.20%; while the recovery of total triterpenoids was 46.15%, product purity was 71.86%. [Conclusion] SP825 type macroreticular resin showed better comprehensive absorption property. It can be used to purify the alcohol extract of Xianhe Compound prescripton.