草珊瑚正丁醇部位化学成分及抑菌活性研究

Studies on chemical constituents and antibacterial activity from n-butanol extract of Sarcandra glabra

目的：研究草珊瑚Sarcandra glabra正丁醇部位的化学成分及抑菌活性。方法：采用Diaion HP-20，Sephadex LH-20，MCI CHP-20及硅胶等柱色谱法对草珊瑚正丁醇部位的化学成分进行分离纯化，根据理化性质及现代波谱技术确定化合物的结构。采用滤纸片法，记录化合物对金葡菌株24h抑菌效果。结果：分离鉴定了7个化合物：5，7，3′，4′．四羟基-6-C-β-D-葡萄糖二氢黄酮碳苷（1），山柰酚-3-O-β-D-葡萄糖酸苷（2），嗪皮啶（3），异嗪皮啶（4），异嗪皮啶-7-O-β-D葡萄糖苷（5），山柰酚（6），异美五松针二氢黄酮（7）。其中化合物2，5，6对金葡菌株的抑菌环直径分别为：（14．67±0．08），（11．14±1．06），（8．26±1．26）mm，化合物4对金葡菌株无抑菌活性。结论：化合物1～3，5为首次从该植物中分离得到。

Objective： To study the chemical constituents and the antibacterial activity from n-butanol extract of Sarcandra glabra. Method： The compounds were isolated by Diaion HP-20, Sephadex LH -20, MCI CHP -20 and silica gel column chromatographic methods. Their structures were identified on the basis of physicochemical and spectroscopic analysis. The antibacterial effect of the compounds were measured against Staphylococcus aureus by filterpaper slice method, finally the antibacterial ring in each group was recorded after 24 hours. Result： Seven constituents were isolated and elucidated as 5, 7, 3′, 4′-tetrahydroxy-6-C-β-D-glucopyranosylflavanone （ 1 ）, kaempferol-3-O-β-D-glucuronide （ 2 ）, fraxidin （ 3 ）, isofraxidin （ 4 ）, isofraxidin-7-O-β-D-glucopyranoside （ 5 ）, kaempferol （6）, pinostrobin （7）. Diameters （ in mm） of antibacterial ring in the compounds 2, 5, 6 were orderly recorded as follows ： 14. 67 ± 0. 08, 11.14 ±1.06, 8.26 ± 1.26 and the compound 4 is not effective. Conclusion： Compounds 1-3 and 5 were isolated from S. glabra for the first time.