人参皂苷Rg_1对PD模型大鼠纹状体多巴胺含量的影响及其机制探讨

Effect and mechanism on dopamine contents of striatum in rat model of Parkinson's disease ginsenoside Rg_1

目的:研究人参皂苷Rg1对6-羟基多巴(6-OHDA)制备的去卵巢(OVX)帕金森病(PD)模型大鼠纹状体(Str)多巴胺(DA)含量的影响及其机制探讨。方法:应用6-OHDA制备OVX的PD模型大鼠,腹腔注射Rg1(10 mg.kg-1)或侧脑室同时给予雌激素受体(ER)拮抗剂ICI 182,780(2.4 ng.kg-1),连续用药14 d,观察阿朴吗啡诱导的大鼠旋转行为。应用高效液相色谱法(HPLC)检测纹状体(Str)内DA及其代谢产物二羟基苯乙酸(DOPAC)和高香草酸(HVA)含量的变化。结果:人参皂苷Rg1可明显改善PD模型大鼠单侧旋转行为(P<0.01),此作用可被ICI 182,780所阻断。Rg1给药组可显著提高损毁侧Str DA及其代谢产物含量(P<0.01)。应用ICI182,780可阻断Rg1的神经保护作用,损毁侧Str DA及其代谢产物含量较Rg1给药组明显降低(P<0.01)。结论:人参皂苷Rg1对6-OHDA所致的去卵巢PD模型大鼠黑质多巴胺能神经元有一定保护作用,此作用与ER途径的激活有关。

Objective： To investigate effect and mechanisms on dopamine contents of striatum （Str） in the 6-OHDA induced rat model of Parkinson＇s disease （PD） by ginsenoside Rg1. Method： Ovariectomized PD rats were treated with vehicle, ginsenoside Rg1 （ 10 mg · kg^-1 ） or estrogen receptor （ER） antagonist ICI 182,780 for 14 d. The change of apomorphine-linduced rotational behavior in PD rats were observed. The high performance lipid chromotophotography （HPLC） was used to determine the contents of DA, dihydroxyphenylacetic acid （DOPAC） and homovanillic acid （HVA）in striatum. Result： Rg1 treatment could ameliorate the PD rat＇s rotational behavior induced by apomorphine （ P 〈 0.01 ）. This effect could be blocked by ER antagonist ICI 182,780. The DA, DOPAC and HVA levels in the injured side of Str for PD rats were significantly decreased compared with the intact side （P 〈 0.01 ）. Rg1 treatment could increase DA contents in the injured side of Str （P 〈 0.01 ）. ICI 182,780 could completely block the neuroprotecfive effects of Rg1. The DA contents and its metabolites in the injured side of the ICI treatment group were significantly decreased compared with the Rg1 group （P 〈0.01 ）. Conclusion： Ginsenoside Rg1 may have protective effects on the dopaminergic neurons for the 6-OHDA induced OVX rat model of PD, ER. May be involved in the protection action.