结晶法分离纯化大豆甾醇中β-谷甾醇和豆甾醇单体

Separation and purification of β-sitosterol and stigmasterol from soybean phytosterol using crystallization

测定β-谷甾醇和豆甾醇在环己酮中的溶解度,利用β-谷甾醇和豆甾醇在环己酮中的溶解度随温度变化的差异,分别用重结晶法提纯β-谷甾醇和豆甾醇,并对豆甾醇的结晶条件(料液比、结晶温度和养晶时间)进行优化。实验结果表明:经过4次结晶操作,β-谷甾醇的纯度可达到90%;豆甾醇结晶过程中采用料液比(混合植物甾醇与环己酮的比,g/mL)为1∶3.3、结晶温度25℃、养晶5h的条件下,经5次结晶豆甾醇的纯度达到96%。

The solubility of β-sitosterol and stigmasterol in cyclohexanone at different temperatures were determined. β-sitosterol and stigmasterol were separated and purified from soybean phytosterol using crystallization based on the difference of variation of the solubilities of stigmasterol and β-sitosterol with temperature changing. The experimental results show that the purity of β-sitosterol is about 90% after the fourth fractional crystallization in cyclohexanene. Some parameters （ratio of material to liquid, crystallization temperature, and ripening time） were optimized for cystallization of stigmasterol as the follows： ratio of material to liquid was1：3.3, crystallization temperature was 25 ℃ and ripening time was 5 h. With above conditions, the purity of stigmasterol is about 96% after the fifth fractional crystallization in cyclohexanene.