杜梨叶片多糖的超声-微波协同萃取法提取

Ultrasonic-microwave synergistic extraction polysaccharide from Pyrus betulaefolia bge. leaf

采用超声-微波协同萃取法和常规水浴提取杜梨叶片多糖,并用蒽酮-硫酸比色法测定多糖含量。结果表明,超声-微波协同萃取法提取杜梨叶片多糖效果更好,两种方法提取多糖的含量分别是21.52%和10.41%;葡萄糖浓度在25.15～100.6μg/mL范围内呈良好的线性关系,平均回收率为100.38%,RSD为1.46%(n=5)。超声-微波协同萃取法可作为杜梨叶片多糖提取的首选方法,蒽酮-硫酸比色法测定多糖含量的方法准确,重复性好。

Ultrasonic-microwave synergistic and water bath were employed to extract the polysaccharide from Pyrus betulaefolia bge. leaf and anthrone-sulphuric acid colorimetry was used to determine its content. There exist remarkably differences between the two methods, ultrasonic-microwave synergistic extraction is the optimal method. The polysaccharide content of the two methods is 20.52% and 10.41% respectively. The linear range was 25.15-100.6 μg/mL for glucose. The average recovery rates 100.38% （RSD 1.46%, n=5）. The ultrasonic-microwave synergistic extraction may take the first choice method. The method of anthrone-sulphuric acid colorimetry determine the polysaccharide content was stable and accurate.