子宫腺肌病的比较蛋白质组学研究

Comparative proteomics analysis of human adenomyosis

目的建立子宫腺肌病病灶组织的蛋白质表达图谱，筛选并鉴定差异表达的蛋白质。方法收集2007年1月至10月北京协和医院妇产科收治的因子宫腺肌病行子宫全切除术患者的子宫肌层病灶组织（腺肌病组）及因宫颈上皮内瘤变和宫颈癌行子宫全切除术患者的正常子宫肌层组织（对照组）各5份，采用双向凝胶电泳技术分别建立两组的蛋白质表达图谱；利用图像分析软件进行比较分析，寻找差异表达的蛋白质点；应用基质辅助激光解吸电离飞行时间质谱和生物信息学方法鉴定差异表达的蛋白质，并进行功能分析。结果子宫腺肌病组织考马斯亮蓝染色图谱中平均含（512±36）个蛋白质点，以其中1块凝胶为参考胶进行匹配，不同凝胶之间蛋白质点的匹配率为83．7％；银染图谱中平均含（762±54）个蛋白质点，不同凝胶之间蛋白质点的匹配率为81．1％。与对照组比较，腺肌病组中有15个恒定差异表达的蛋白质点，其中10个被成功鉴定。这些蛋白质的功能涉及细胞骨架、氧化反应、凋亡和免疫反应等。结论子宫腺肌病组织在细胞骨架、氧化反应、凋亡、免疫反应等过程中存在异常，这些过程可能参与了子宫腺肌病的发病。

Objective To set up the proteomic protein profiling of adenomyotic tissue and normal uterine muscle and identify the abnormally expressed proteins in adenomyotic tissue. Methods Samples of adenomyotic tissue （adenomyosis group） and age-matched healthy uterine muscle （control group） were collected from totally 10 patients undergoing transabdominal hysterectomy for adenomyosis and cervical diseases at Peking Union Medical College Hospital from January 2007 to October 2007. The proteomics profiling of adenomyotic tissue and normal uterine tissue were established using two dimensional gel electrophoresis （2-DE） and gel staining method. The differently expressed protein spots were detected by gel comparison using image analysis software and identified using matrix-assisted laser desorption/ionization time of flight mass spectrometry （ MALDI-TOF-MS）. Results In Coomassie blue stained gels there were on average （512 ±36） spots and compared with the reference gel the matching rate was 83.7%. In silver stained gels there were （762 ±54） spots and compared with the reference gel the matching rate was 81.1%. Compared with normal uterine muscle, there were 15 protein spots disregulated in adenomyotic tissue. Among them 10 protein spots were successfully identified by mass spectrometry. The functions of these disregulated proteins included cell skeleton, oxidation, apoptosis and immune reaction. Conclusions Comparative proteomics analysis is a useful approach for the study of adenomyosis. Compared with normal uterine muscle there are abnormalities in cell skeleton, oxidation, apoptosis and immune reaction. These life processes may participate in pathophysiology of adenomyosis.