盐酸吉西他滨与5-氟脲嘧啶在鼠晚期肺癌移植瘤的疗效比较

Comparison of the therapeutic effects of 5-FU with gemcitabine on advanced lung cancer in mice model

目的建立晚期肺癌小鼠动物模型,比较5-氟脲嘧啶(5-FU)与盐酸吉西他滨在该模型中的疗效。方法通过胸腔移植的方法建立60只晚期肺癌的小鼠动物模型,随机分为对照组(A组)、5-FU组(B组)和盐酸吉西他滨组(C组),每组20只。记录接受5-FU与盐酸吉西他滨治疗后的体重变化及饲料消耗情况,并用免疫组化方法检测肿瘤组织内增殖细胞核抗原(PCNA)和血管内皮细胞因子(VEGF)的蛋白表达。结果治疗后,C组和B组小鼠体重较对照组显著增加(P<0.05),C组明显重于B组(P<0.05)。B组及C组的饲料消耗量明显高于对照组(P<0.05或P<0.01),C组显著高于B组(P<0.01)。C组肿瘤组织PCNA、VEGF阳性细胞数明显低于B组及A组(P<0.01和P<0.05)。结论在晚期肺癌动物模型中,盐酸吉西他滨疗效优于5-FU。

Objective To compare the therapeutic effects of 5-FU with gemcitabine（GEM）on advanced Lewis lung carcinoma in mice model. Methods The advanced lung cancer model was established in mice by implanting the subculturing Lewis lung cancer cells into the thoracic cavity of mice. Sixty mice were randomly divided into three groups of 5-FU reatment （group 5-FU）, GEM treatment （group GEM） and control （group C） with 20 mice each. The weight of the mice and the consumption of feed were recorded daily. The expressions of proli-ferating cell nuclear antigen （PCNA） and vascular endothelial grouth factor（VEGF） in the lung cancer tissues were detected by immunohistochemical method. Results After treatment, the body weights of mices in group GEM and 5-FU were significantly higher than those in group C （P〈0.05）, which were higher in group GEM than those in group 5-FU （P〈0. 05）. The food consumption in group GEM and 5-FU was more than that in group C （P〈0. 05） and P〈0.01） ,which was more in group GEM than that in group 5-FU （P〈0.01）. The expressions of PCNA and VEGF in group GEM were higher than those in group 5-FU and C （P〈0.01 and （P〈0. 05）. Conclusion The therapeutic effect of GEM on the implated advaneed lung cancer is superior to 5-FU in mice.