应用荧光原位杂交技术定位水稻的端粒相关序列和BAC克隆

Localization of the Telomere-Associated-Sequence and a Bacterial Artificial Chromosome in Rice by FISH

用ＰＣＲ扩增得到的水稻端粒相关序列（ＴＡＳ）Ｔａｓ３和一个ＢＡＣ克隆（ＢａｃｔｅｒｉａｌＡｒｔｉ－ｆｉｃｉａｌＣｈｒｏｍｏｓｏｍｅ，ＢＡＣ）为探针，与间期核和中期细胞染色体进行荧光原位杂交（Ｆｌｕｏ－ｒｅｓｃｅｎｃｅＩｎＳｉｔｕＨｙｂｒｉｄａｔｉｏｎ，ＦＩＳＨ），确定了其在染色体上的位置。

Telomere-Associated-Sequence (TAS) and a BAC (Bacterial Artificial Chromosome) clone in interphase nuclei and metaphase cell are identified by the technique of Fluoresence In Situ Hy- bridization (FISH), which has been widely applied in analysis of chromosome structure and con- struction of DNA molecular physical maps.