摘要
Young leaves and shoot with Apple stem pitting virus（ASPV） detected by RT-PCR（reverse transcription-polymerase chain reaction） from Korla pear were used as materials and total RNA was extracted.Two expected fragments had been obtained by RT-PCR with two specific pairs of primers based on the sequence of NC003462 published in GenBank.The specific fragments had been recovered and cloned,and the white colonies had been screened out.After enzyme digestion of the plasmids isolated,the positive clone was selected to be sequenced.Compared with NC003462,three ORFs（open reading frame）-ORF2,ORF3 and ORF4 of ASPV with 673 bp,365 bp,276 bp representing homology of 77%,88.52%,86.33% respectively had been obtained.
Young leaves and shoot with Apple stem pitting virus （ASPV） detected by RT-PCR （reverse transcription-polymerase chain reaction） from Korla pear were used as materials and total RNA was extracted. Two expected fragments had been obtained by RT-PCR with two specific pairs of primers based on the sequence of NC_003462 published in GenBank. The specific fragments had been recovered and cloned, and the white colonies had been screened out. After enzyme digestion of the plasmids isolated, the positive clone was selected to be sequenced. Compared with NC_003462, three ORFs （open reading frame） -ORF2, ORF3 and ORF4 of ASPV with 673 bp, 365 bp, 276 bp representing homology of 77%, 88.52%, 86.33% respectively had been obtained.
出处
《植物病理学报》
CAS
CSCD
北大核心
2008年第4期433-435,共3页
Acta Phytopathologica Sinica
基金
国家自然科学基金资助项目(30360066)
国家科技攻关计划引导项目(2003BA546C)
兵团科委项目(NKB02SDXNK01SW)
