三七总皂苷、三七皂苷R1、人参皂苷Rb1、人参皂苷Rg1对光化学反应诱导的大鼠肠系膜细静脉血栓的抑制作用——三七主要成分抑制细静脉血栓

Attenuation Effects of PNS,R1,Rb1,and Rg1 on Venous Thrombosis Induced in Rat Mesentery by Photochemical Reaction.

目的:三七总皂苷(PNS)、三七皂苷R1(R1)、人参皂苷Rb1(Rb1)、人参皂苷Rg1(Rg1)是三七的主要水溶性成份。含有PNS、R1、Rb1、Rg1的中药复方制剂复方丹参滴丸(CP)能抑制光化学反应诱导的大鼠肠系膜细静脉血栓的形成。但是有关PNS、R1、Rb1、Rg1对血栓形成的在体影响尚不清楚。本研究探讨PNS、R1、Rb1、Rg1对光化学反应(PR)诱导的大鼠肠系膜细静脉血栓形成的抑制作用。方法:将麻醉的雄性SD大鼠(200～250g)的肠系膜展开至观察板上,经大鼠股静脉注入血啉甲醚(HMME,1mg/ kg),用倒置荧光显微镜100瓦汞灯作光源经蓝光滤光片照射在选定的大鼠肠系膜细静脉上。在照射开始时,设定显示器的时间为0分,在血栓达到细静脉管径的1/2时停止照射,通过连接在显微镜上的CCD连续观察并记录30min内细静脉血栓出现的时间、血栓到达细静脉直径一半的时间、血栓与照射的血管面积比。一部分大鼠在光化学反应前10min,经颈静脉分别一次性注入PNS、R1、Rb1、Rg1(各10mg/kg.BW)、或阿斯匹林(ASP,200mg/kg.BW)提前60min灌胃。结果:光化学反应组在照射13.67±2.67s后细静脉开始出现血栓,血栓与血管径的面积比迅速增加。PNS、R1、Rb1、Rg1、ASP可以延长由照射开始到血栓出现的时间,减少血栓的面积。结论:PNS、R1、Rb1、Rg1可以抑制光化学反应后细静脉血栓的形成,其效果与ASP近似。

Panax notoginseng saponins （PNS）, Notoginsenoside R1 （R1）, Ginsenoside （Rb1）, and Ginsenoside Rg1 （Rg1） are the aqueous extracts of Panax notoginseng （Sanqi）. Cardiotonic pill （CP）, made up of traditional Chinese medicinal elements, such as PNS, R1, Rb1, and Rg1, is found to inhibit venular thrombosis induced by photochemical reaction （PR） in rat mesentery. The present study utilizes a microcirculation dynamic viewing system to understand the inhibiting effects of PNS, R1, Rb1, and Rg1 on thrombosis induced by PR in rat mesentery. Initiation time and half-size time of thrombus, and area ratio of thrombus/venule are determined using an inverted microscope and high speed video camera system. Photochemical reaction in rat mesentery venule induces changes in rat mesentery microcirculation, leading to thrombus initiation, a significant increase in thrombus area. Study results show that PNS, R1, Rb1, and Rg1 are able to delay thrombus initiation time, and inhibit venous thrombosis induced by PR.