养血清脑颗粒对双侧颈动脉结扎-再通引起的蒙古沙鼠脑皮层微循环障碍和脑灌流量的改善作用

The Attenuation Effects of Cerebralcare Granule~ on the Microcirculatory Disturbance in Mongolia Gerbil Brain Induced by Occlusion of Carotid Arteries and Reperfusion

目的:研究养血清脑颗粒(Cerebralcare Granule,CG)对双侧颈动脉结扎再通引起的蒙古沙鼠脑皮层微循环障碍和脑灌流量的改善作用。方法:采用蒙古沙鼠双侧颈动脉缺血再灌注(is—chemia/erperfusion,I/R)模型,在正立显微镜微循环观察系统下,观察脑皮层细静脉管径、细静脉红细胞流速、dihydrorhodamine 123(DHR)萤先强度和血浆白蛋白漏出的变化,记录脑表面血流量。一部分沙鼠在缺血前90min灌胃给予CG0.8 g/kg。结果:I/R 30min后,粘附于细静脉壁的白细胞数量、细静脉过氧化物、血浆白蛋白漏出显著增加,脑血流量降低。CG可以显著地抑制上述变化。结论:CG前给药可以抑制双侧颈动脉结扎再通引起的脑微循环障碍和脑灌流量,该作用可能与抑制过氧化物产生有关。

This study investigates the attenuation effects of Cerebralcare Granule （CG） on microcirculatory disturbance in gerbil brain induced by occlusion of carotid arteries and reperfusion. In the study, an ischemia and reperfusion （I/R） injury model was created by occluding bilateral carotid arteries and releasing in Mongolian gerbils. Cerebral venular diameters, RBC velocity, number of adherent leukocytes, fluorescence intensity of dihydrorhodamine 123 （DHR）, and albumin leakage were measured under an upright biological microscope microcirculatory observing system. Cerebral blood flows was also measured. Some gerbils were orally administrated with CG （0.8g/kg.bw） 90 min before I/R. Study results show that I/R increases adherent leukocytes to venular wails, DHR fluorescence intensity in venular wails, as well as albumin leakage, and results in a declined cerebral blood flow 30 min after reperfusion. Pretreatment with CG alleviates these symptoms significantly. It is believed that CG pretreatment may attenuate the cerebral microcirculatory disturbance induced by I/R. The efficacy of CG is probably associated with its potential of scavenging reactive oxygen species.