丹参提取物F对乙醇诱导的大鼠胃粘膜损伤和胃粘膜微血管损伤的抑制作用及对脂质过氧化物的影响

Inhibitory Effects of Dan Shen Extract F on Ethanol-Induced Gastric Mucosal Lesion and Gastric Mucosal Blood Vessels Impairment and Its Effects on Lipid Peroxidation in Rats

目的:观察丹参提取物F(DSE—F)对乙醇诱导的大鼠胃粘膜损伤及其微血管损伤的抑制作用及对脂质过氧化物的影响。方法:160—200g Wistar系雄性大鼠随机分为空白对照,对照及丹参提取物F组,实验前24hr禁食不禁水,经胃管向胃内分别灌注生理盐水、无水乙醇、及DSE—F(1mL/100g),0.5hr后处死动物。测定了胃粘膜损伤、胃粘膜微血管的损伤、胃粘膜超氧化物歧化酶(SOD)活性和谷胱甘肽过氧化物酶(GSH-Px)活性及丙二醛(MDA)含量。结果:1)对照组及DSE—F组的大鼠胃粘膜损伤面积(%)分别为7.57±0.50,2.53±0.22;光镜下Ⅲ级损伤(%)分别为51.88±11.20,20.67±5.05;两组差异均有统计学意义(P<0.01),DSE—F对乙醇致胃粘膜损伤具有抑制作用。2)对照组及DSE—F组的胃粘膜伊文斯蓝EB含量(ng/g湿重)分别为12.46±0.70,8.05±1.86;单星蓝MB染色面积(%)分别为4.54±0.83,0.90±0.10;两组差异均有统计学意义(P<0.05),DSE—F可减轻乙醇致胃粘膜微血管的损伤作用。3)与空白对照组的大鼠胃粘膜的SOD(Nu/mg pro)和GSH—Px(u)的活性分别为7.68±0.68(Nu/mg pro),52.67±21.32(u)相比,对照(无水乙醇)组的SOD及GSH—Px的活性为3.18±0.59(Nu/mg pro),31.52±14.65(u),均显著降低(P<0.01),DSE—F组的SOD及GSH—Px的活性为6.11±0.54(Nu/mg pro),49.31±15.82(u)均显著高于对照组(P<0.01),而空白对照组的MDA含量(nmol/mg pro)为0.90±0.05,对照组及DSE—F组的MDA含量则分别为1.38±0.19,0.92±0.06。结论:丹参提取物F具有对乙醇致大鼠胃粘膜损伤及胃粘膜微血管损伤的抑制作用,该作用与其促进胃粘膜SOD及GSH-Px的活性及抑制MDA的作用相关。

We investigated the inhibitory effects of Dan Shen extract F on ethanol-induced gastric mucosal injury and microvascular injury, and its effects on lipid peroxidation in rats. In the study, 160-200 g Wistar male rats were randomly divided into different groups, including blank control, control, and Dan Shen extract F. A 24-hr fasting was arranged before the experiment, though water was allowed. Rats were fed with saline, ethanol, and DSE-F （1mL/100g） respectively, before being killed 0.5 hour later. Gastric mueosal lesion, gastric mueosal mierovaseular injury, gastric activity of superoxide dismutase （SOD）, and glutathione peroxidase （GSH-Px） activity and content of gastric mucosal malondialdehyde （MDA） were measured. In comparison with the control group, the DSE-F group showed a reduced gastric mueosal lesion （2.53 ±0.50 7.57 ± 0.22, P〈 0.01）, and a reduced gastric mueosal injury depth （Class Ⅲ injury were 20.67±11.20 vs 51.88 ± 5.05, P〈 0.01）. EB in the gastric mueosa reached 8.05±1.86 vs 12.46±0.70 （ng/g wet weight） （P〈0.05）, with the MB stain area being at 0.90±0.10 vs 4.54±0.83（%） respectively （P〈0.05）. In addition, the control group showed a reduced SOD （Nu / mg pro） with GSH-Px （u） activity of gastric mueosa at 3.18±0.59 vs 7.68± 0.68 （Nu /mg pro, P〈0.01）, 31.52±14.65 vs 52.67±21.32（u, P〈0.01）, compared with the blank control group. However, the SOD and GSH-Px activity in gastric mueosa of the DSE-F group accounted for 6.11 ±0.54 （Nu/mg pro） and 49.31±15.82（u） respectively, which was significantly higher than the control group （P 〈0.01）. Comparing with the control group, the DSE-F group had a gastric mueosal MDA content of 0.92±0.06 vs 1.38±0.19 （nmol/mg pro, P〈0.05）. It is believed that DSE-F has an inhibitory effect on gastric mueosal lesion and mierovaseular injury induced by ethanol, which is associated with its role in promoting the gastric mueosal SOD and GSH-Px activity and reducing the content of gastric mueosal MDA.