氧化应激反应在过度训练致急性肾损伤中的作用及山莨菪碱、旋覆花素、苦碟子的影响

The Effects of Oxidative Stress on Overtraining-Induced Acute Kidney Injury in Rats and the Protective Effects of Anisodamine,Inula Britannica,and Ixeris Sonchifolia Hance on Kidney

目的:过度训练可引起急性肾损伤(OTIAKI),为了解氧化应激反应在过度训练致急性肾损伤中的作用,本文研究了过度训练大鼠血清及肾组织SOD和MDA的改变,观察了山莨菪碱、旋覆花素、苦碟子对上述变化的影响。方法:将80只雄性Wistar大鼠随机分为安静对照组(CN)、力竭运动组(ES)、山莨菪碱干预组(AD)、旋覆花素干预组(IB)、苦碟子干预组(IS)。采用大鼠游泳至力竭建立过度训练致急性肾损伤模型。采用黄嘌呤氧化酶法检测血清和肾组织SOD和MDA的改变。结果:力竭后即刻和力竭后6h大鼠血清和肾组织SOD明显降低(P<0.05),MDA明显升高(P<0.05);血清SOD和MDA两个指标于力竭后24h恢复达到对照组水平,肾组织SOD和MDA与力竭后6h比较均有所恢复,但未达到对照组水平。于力竭后6h,山莨菪碱、旋覆花素及苦碟子三种药物对过度训练引起的大鼠血清和肾组织SOD活性的降低和MDA含量的增高均具有不同程度的逆转作用;于力竭后24h,三种药物组大鼠血清和肾组织SOD和MDA均恢复到对照组水平。结论:氧化应激反应主要参与力竭后早期引起的肾损伤过程,山莨菪碱、旋覆花素及苦碟子在OTIAKI早期可通过抗氧化应激反应来发挥肾脏保护作用。

We studied the effects of oxidative stress on overtraining-induced acute kidney injury （OTIAKI）, and the possible protective effects of Anisodamine, Inula Britannica, and Ixeris Sonchifolia Hance on kidney. In the study, eighty male Wistar rats were randomly divided into five groups： control group （CN, n=8）, exhaustive swimming （ESI, ES6h, ES24h, n=24）, Anisodamine （AD6h, AD24h, n=16）, Inula Britannica （IB6h, IB24h, n=16）, and Ixeris Sonchifolia （IS6h, IS24h, n=16）. An overtraining-induced acute kidney injury model was established for exhaustive swimming rats. The variations of SOD and MDA in serum and renal tissues were measured in the overtrained rats. SOD decreased, while MDA increased significantly in both serum and renal tissues of ESI and ES 6 h rats （p 〈0.05）. The serum SOD went up to CN levels, while MDA went down to CN levels in ES 24 h rats. Renal tissue SOD saw an increase, though remaining lower than CN. Meanwhile, renal tissue MDA dropped, though remaining higher than CN levels. Compared with CN, the level of SOD in serum increased significantly in AD 6 h, IB 6 h, and IS 6 h rats, while the level of MDA in serum decreased significantly in AD 6 h, IB 6 h, and IS 6 h rats （p 〈0.05）. The level of SOD in serum lifted to CN levels, while the level of MDA in serum dropped down to CN levels at 24 h （AD, IB and IS rats）. The level of SOD in renal tissue went up noticeably at 6 h in AD, IB and IS rats （p〈0.05）, with an especially large increase in AD rats. MDA decreased significantly in renal tissues at 6 h in AD, IB, and IS rats（p〈0.05）, with an especially large increase in AD and IS rats. Both SOD and MDA in renal tissues returned to CN levels at 24 h in AD, IB, and IS rats. Study results suggest that overtraining could increase oxidative stress in the early stage after an exhaustive swimming. Anisodamine, Inula Britannica, and Lxeris Sonchifolia Hance are able to produce an anti-oxidative stress effect on overtrained rats.