重组LIGHT-Fc基因对食管鳞癌Eca109细胞生长的抑制

Inhibitory effect of recombinant LIGHT-Fc gene on the proliferation of human esophageal squamous carcinoma cell line Eca109

目的研究重组LIGHT—Fc基因对食管鳞癌Eca109细胞的抑制效应。方法以脂质体介导LIGHT—Fc基因转染食管鳞癌Eca109细胞，观察基因转染后对Eca109细胞体外生长的影响。建立荷瘤裸小鼠模型，分为野生型组（Eca109／Wt）、转空质粒组（Eca109／neo）和实验组（Eca109／LIGHT），每组15只。观察各组成瘤情况和瘤组织的病理表现。结果Eca109细胞中有LIGHT受体的表达，转染LIGHT—Fc基因后，瘤细胞的生长被抑制。野生型组、转空质粒组和实验组成瘤分别为12、11和5只，实验组与其余两组比较差异有统计学意义（X^2=6．652，4．821，P〈0．05）。病理检查显示在实验组小鼠瘤组织中存在较为明显的坏死。结论LIGHT—Fc基因的表达对食管鳞癌Eca109细胞具有体内和体外抑制作用。

Objective To investigate the inhibitory effect of recombinant LIGHT-Fc gene on the proliferation of human esophageal carcinoma cell line Eca109. Methods LIGHT-Fc expression vector was transfected into human esophageal squamous carcinoma cell line Eca109 by using DOTAP liposomal transfection reagents. The effects of LIGHT-Fc gene on the proliferation of esophageal carcinoma cell line Eca109 in vitro were detected by cell growth curve and MTT assay, Forty-five nude mice were equally divided into Eca109/Wt group, Eca109/neo group and Eca109/LIGHT group. Carcinogenesis and pathological expression of the esophageal carcinoma tissues were observed. Results The expressions of LIGHT receptors were detected in Eca109 cells. The proliferation of Eca109 cells was inhibited after trasfecting LIGHT-Fc gene into Eca109 cells. The numbers of tumors generated in Eca109/Wt group, Eca109/neo group and Eca109/LIGHT group were 12, 11 and 5, with statistical significance between Eca109/LIGHT group and the other two groups （ X^2 = 6. 652, 4. 821, P 〈 0.05 ）. The result of histopathological examination indicated that the tissue necrosis appeared significantly in tumors derived from Eca109/LIGHT cells. Conclusions The growth of esophageal squamous carcinoma cell line Eca109 can be suppressed by LIGHT-Fc gene whether in vitro or in vivo.