摘要
背景与目的:研究Bid在维生素E琥珀酸酯(vitamin E succinate,VES)诱导人胃癌SGC-7901细胞凋亡中的作用。材料与方法:在VES诱导SGC-7901细胞凋亡过程中,检测Bid蛋白的活化;用RNA瞬时干扰阻断Bid的表达,再用VES作用SGC-790细胞后,比较Bid阴性表达和Bid阳性表达细胞的凋亡率、线粒体膜电位(ΔΨm)、胞浆中细胞色素C含量和PARP活化程度。结果:VES作用细胞后Bid发生活化;Bid阴性表达细胞的凋亡率较Bid阳性表达细胞降低,线粒体膜电位升高,胞浆中细胞色素C含量减少,PARP活化减弱。结论:在VES诱导SGC-7901细胞凋亡过程中,Bid可能发挥重要作用。
BACKGROUND AND AIM： To study the roles of Bid in vitamin E succinate （VES）-induced apoptosis of human gastric carcinoma SGC-7901 cells. MATERIALS AND METHODS： The activation of Bid was detected by western blot. Bid was blocked by transient RNA interference （RNAi）, and apoptotic rate, mitochondrial transmembrane potential （△Ψm）, the amount of cytosolic cytochrome C and the level of PARP cleavage were determined in Bid-negative cells and Bid-positive cells. RESULTS： Bid was activated by VES. Compared with Bid-positive cell, VES caused lower apoptotic rate, higher mitochondrial transmembrane potential, decreased release of cytochrome c to cytosol and lower level of PARP cleavage in Bid-negative cells. CONCLUSION： This study suggested Bid might play an important role in VES-induced apoptosis of human gastric carcinoma SGC-7901 cells.
出处
《癌变．畸变．突变》
CAS
CSCD
2008年第4期258-261,共4页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
国家自然科学基金资助项目(30671757)
