三丁基锡引起人羊膜细胞氧化损伤及DNA损伤的研究

Study of Oxidative Damage and DNA Damage in FL Cells Induced by Tributyltin

背景与目的:研究三丁基锡(tributyltin,TBT)对人羊膜细胞FL(human amnion cells)氧化损伤和DNA损伤的诱导作用。材料与方法:将不同浓度TBT(0、2、4、6、8、10μmol/L),分别对FL染毒2h和4h,各染毒组同时设不加TBT的对照组,染毒后分别用MTT法检测TBT对FL细胞增殖率的影响,用DCFH-DA法检测FL细胞活性氧自由基(ROS)水平,用彗星实验检测TBT对FL细胞DNA的损伤。结果:TBT对FL细胞染毒4h时,其2、8、10μmol/L浓度组的细胞增殖率较对照组显著下降(P<0.05,P<0.01,P<0.001),且随TBT浓度升高而增殖率呈下降的趋势。TBT3、4μmol/L染毒组FL细胞的ROS水平较对照组升高,且4μmol/L染毒组与对照组比较差异具有统计学意义(P<0.05)。在TBT2、3、4μmol/L染毒组随着TBT浓度的升高,FL细胞核尾长、尾相均显著升高(P均<0.05)。结论:TBT可引起FL细胞的氧化损伤及DNA损伤。

BACKGROUND AND AIM： The study was undertaken to assess the oxidative stress and DNA damage in human amnion cells induced by TBT. MATERIALS AND METHODS： Cultured FL cells were exposed to different concentrations of TBT （0, 2, 4, 6, 8, 10 μmol/L） for different durations （2 h and 4 h）. Cell proliferation rate was evaluated by MTr assay. After exposure to different concentrations of TBT （0,1,2,3,4μmol/L） for 2 h,the ROS level and DNA damage of FL cells were measured by DCFH-DA method and the single cell gel electrophoresis method, respectively. RESULTS： After exposure to different concentrations of TBT for 4 h, the FL cell proliferation rates in the 2, 8, 10μmol/ L groups were significantly decreased as compared to control（P〈0.05, P〈0.01, P〈0.001） in a dose-dependent manner. The ROS level was increased in the 3 and 4 μmol/L groups in the dose-dependent manner and the difference was significant at 4μmol/L group compared to the control（P 〈0.05）. Meanwhile, TBT exposure led to the interrelated increase of nucleus tail length and tail moment in a dose- dependent manner and the differences were significant at 2, 3 and 4μmol/L group compared to the control（P 〈0.05） . CONCLUSION： TBT exposure could cause oxidative stress and DNA damage to FL cells.