β-胡萝卜素对丝裂霉素C致小鼠骨髓细胞遗传损伤的保护作用

Effect of β-carotene on Genetic Damage Induced by Mitomycin-C in Bone Marrow Cells of Mice

背景与目的：探讨β-胡萝卜素（β-carotene，pc）对丝裂霉素C（mitomycin-C，MMC）诱导的小鼠骨髓细胞遗传损伤的保护作用。材料与方法：分别用3mg／kg和30mg／kg的pc连续饲喂小鼠8d后，以2mg／kgMMC进行腹腔注射染毒，另设阳性对照组（用生理盐水）和阳性对照组（一次性腹腔注射MMC2mg／kg）。各组于末次给药后12、24h处死小鼠，分别采用单细胞凝胶电泳技术和染色体畸变分析检测骨髓细胞DNA损伤及染色体畸变情况。结果：两个不同剂量的pc与MMC联合作用组小鼠骨髓细胞拖尾率、平均尾长及染色体畸变率均低于阳性对照组（单独MMC），但均高于阴性对照组，差异均有统计学意义（P〈0．01）。结论：pc对MMC诱导的小鼠骨髓细胞遗传损伤有明显的保护作用，但在实验所给剂量范围内（3—30mg／kg）并不能完全抑制MMC所造成的遗传损伤。

BACKGROUND AND AIM： To assess the protective effects of β-carotene（βC） on DNA damage induced by mitomycin-C （MMC） in bone marrow cells of mice. MATERIALS AND METHODS： Two batches of mice were primed orally with βC in concentrations of 3 and 30 mg/kg for 8 days and then followed by MMC intraperitoneal injection at dose of 2 mg/kg on day 9. DNA and chromosome damage were examined by single cell gel electrophoresis（SCGE） and chromosome aberrations analysis. RESULTS： The comet frequency, tail length and the chromosomal aberration rate of two doses of ~C were significantly lower than those of the MMC group. CONCLUSION： To some extent, βC could protect the bone marrow cells of mice from damage induced by MMC in vivo.