HSA-sTRAIL融合蛋白的表达质粒pPICZαA-HSA-sTRAIL的构建

Construction of pPICZαA-HSA-sTRAIL eukaryotic expression plasmid

目的:构建pPICZαA-HSA-sTRAIL真核表达质粒。方法:用PCR方法扩增sTRAIL基因,然后克隆入pPICZαA载体,构建pPICZαA-sTRAIL质粒,然后将HSA基因克隆入pPICZαA-sTRAIL质粒,构建pPICZαA-HSA-sTRAIL真核表达质粒。重组子均进行酶切鉴定和PCR鉴定,最后进行DNA测序验证。结果:PCR方法克隆出目的基因,并成功构建了pPICZαA-HSA-sTRAIL质粒。酶切鉴定、PCR鉴定和DNA测序均证明构建的表达质粒完全与预期一致。结论:成功构建了pPICZαA-HSA-sTRAIL真核表达质粒。

Objective：To Construct the pPICZαA -HSA -sTRAIL eukaryotic expression plasmid. Methods： The DNA of sTRAIL was amplified by PCR and cloned into pPICZαA vector. The DNA fragment of HSA was amplified and cloned into pPICZαA -sTRAIL to construct pPICZαA -HSA -sTRAIL. Double restriction endoenzyme digestion and PCR were performed in comfirmation of recombinants, and the recombinant DNA was sequenced. Results：The target DNA of sTRAIL and HSA were amplified by PCR, and the plasmid of pPICZαA - HSA -sTRAIL was constructed successfully. The recombinant plasmid was confirmed by digestion, PCR and DNA sequencing. Condusion：pPICZαA -HSA -sTRAIL eukaryotic expression plasmid was constructed successfully.