慢性髓系白血病骨髓间充质干细胞整联蛋白表达变化研究

Expression of Integrins in Bone Marrow Mesenchymal Stem Cells Derived from Patients with Chronic Myeloid Leukemia

为研究慢性期慢性髓系白血病(CML)患者骨髓间充质干细胞(BMMSC)的生长活性及其整联蛋白(integrins)的表达水平,并探讨BMMSC在CML发病中的作用,以慢性期CML患者为实验组,健康人为对照组,抽取骨髓液分离单个核细胞行BMMSC体外培养,动态观察细胞形态并进行计数、绘制生长曲线;取第3、4代BMMSC提取总RNA,逆转录,real-time PCR法检测integrin mRNA表达水平。结果表明:慢性期CML患者BMMSC生长活性较健康人无明显差异;实验组BMMSC表达integrin mRNA水平较对照组显著升高(p<0.05)。结论:BMMSCs中integrin高表达影响CML发病。

The purpose of this study was to investigate the growth characteristics and the expression level of integrin mRNA of the cultured bone marrow mesenchymal stem cells （BMMSCs） from patients with chronic myeloid leukemia （CML） in myeloid crisis （MC）, and explore the role of BMMSCs in pathogenesis of CML. Five CML patients were enrolled in experimental group, five healthy persons were used as control. BMMSCs were cultured in vitro. The morphology of BMMSCs was observed every day and the growth curve were portrayed, and the ability of cell proliferation were detected according to the daily results of cell counting. Total RNA was extracted from third and fourth passages of BMMSCs, The expression of integrins mRNA of BMMSCs were measured by real-time PCR. The results showed that the BMMSCs of experimental and control groups had no difference in growth characterisctics, but the expression of integrins mRNA of the BMMSCs was higher in CML patients than in normal control group （p 〈0.05 ）. It is concluded that the abnormally high expression of integrins of BMMSC from the CML patients take part in pathogenesis of CML.