多发性骨髓瘤细胞DNA含量和细胞周期分析

DNA Content and Cell Cycle Analysis of Myeloma Cells in Patients with Multiple Myeloma

本研究探讨MM的遗传学背景和细胞增殖特点。应用直接免疫磁珠法分选19例多发性骨髓瘤(MM)患者的骨髓瘤细胞,用流式细胞术检测骨髓瘤细胞的DNA含量和细胞周期。结果表明:19例患者中4例骨髓瘤细胞为超二倍体,15例骨髓瘤细胞均为二倍体;正常人浆细胞处于S+G2/M期细胞的比例为(1.15±0.60)%,MM患者骨髓瘤细胞处于S+G2/M期细胞比例为(10.06±12.60)%,两组相比具有显著性差异(p=0.001)。初治组患者出现超二倍体比例为11.76%,复治组患者为100.00%,两组相比有显著性差异(p=0.035);初治组骨髓瘤细胞处于S+G2/M期的比例为(7.12±4.98)%,复治组为(35.10±32.56)%,两组相比有显著性差异(p=0.001)。结论:MM患者骨髓细胞DNA含量和细胞周期分布的变化提示了该病遗传背景的复杂性和细胞增殖的异常,而此与病程可能具有一定的相关性。

The study was aimed to investigate the genetic backgroud and proliferation characteristics of multiple myeloma （MM）. Myeloma cells were isolated from bone marrow of 19 MM patients by direct immunomagnetic cell sorting and the DNA content and cell cycle analysis were carded out by flow cytometry. The results showed that in 4 patients the myeloma cells were found to be hyperdiploid and in 15 patients those were found to be diploid respectively by DNA content analysis; the proportion of plasm cells from normal controls in S ＋ G2/M phase was （ 1.15 ± 0.60） %, and that of myeloma cells from MM patients was （ 10.06 ± 12.60） % which was significantly higher than that in the former （p = 0.001 ）. The incidence of hyperdiploid in newly diagnosed patients was 11.76%, and that of treated patients was 100.00% which was significantly higher than that in the former （p = 0.035 ） ; the proportion of myeloma cells from newly diagnosed patients in S ＋ G2/M phase was （7.12 ±4.98 ）%, and that of treated patients was （35.10 ± 32.56）% which was also significantly higher than that in the former （p =0.001 ）. It is concluded that the variety of myeloma cells in DNA content and cell cycle suggests the complicated genetic backgroud and abnormal proliferation of MM, which relate with the course of disease to some extent.