摘要
本研究探讨CCL-2/MCP-1血浆水平在异基因造血干细胞移植前后的变化,以了解该趋化因子在急性移植物抗宿主病(aGVHD)和特发性肺炎综合症(IPS)发生、发展中的作用。选择22例异基因造血干细胞移植病例为研究对象,其中无/度aGVHD 14例,Ⅱ-Ⅳ度aGVHD 8例,在22例中8例临床诊断为IPS。采用固相双抗体夹心酶联免疫吸附方法每周测定血浆CCL-2/MCP-1水平并分析造血干细胞移植前、aGVHD高峰时、aGVHD控制后或进展时(Ⅲ-Ⅳ度者)CCL-2/MCP-1水平与aGVHD之间的关系,同时分析移植过程中CCL-2/MCP-1动态变化和IPS的关系。结果表明,Ⅱ-Ⅳ度aGVHD高峰时血浆CCL-2/MCP-1水平较移植前明显升高(p<0.05),aGVHD被控制时CCL-2/MCP-1水平下降至移植前水平(p<0.05),病情进展时CCL-2/MCP-1持续升高(p<0.05)。发生aGVHD和/或IPS的患者CCL-2/MCP-1水平明显高于既无(或轻度)aGVHD又无IPS的患者(p=0.001)。临床诊断为IPS的患者,在移植过程中CCL-2/MCP-1水平明显升高(p=0.006)。结论:CCL-2/MCP-1血浆水平与aGVHD的发生有一定的相关性,但与IPS的发生明显相关,表明CCL-2/MCP-1在aGVHD及IPS发生中发挥作用。
The aim of this study was to investigate the relationship between the plasma levels of chemokine CCL-2/ MCP-1 and acute graft-versus-host disease （aGVHD） and/or idiopathic pneumonia syndrome （IPS） after allogeneic hematopoietic stem cell transplantation （allo-HSCT）. ELISA assays were used to detect the plasma level of CCL-2/ MCP-1 of 22 patients who received allo-HSCT, including 14 patients without or with grade Ⅰ, 8 patients with grade Ⅱ-Ⅳ aGVHD, respectively. 8 out of 22 patients were also diagnosed with IPS clinically. The dynamic changes of the plasma levels of CCL-2/MCP-1 chemokine and its correlation with aGVHD and/or IPS were analysized retrospectively. The resuits showed that the plasma levels of CCL-2/MCP-1 in the patients with moderate and serious aGVHD （grade Ⅱ-Ⅳ） significantly increased, as compared with that prior to allo-HSCT （p 〈 0.05 ）. The plasma levels of CCL-2/MCP-1 in the patients with aGVHD and/or IPS were higher significantly than those without any of these complications （p = 0. 001 ）. The retrospective analysis indicated that the plasma levels of CCL-2/MCP-1 in the patients with IPS significantly increased （p =0. 006）. It is concluded that plasma level of CCL-2/MCP-1 correlates with aGVHD and/or IPS, and plays a role in the pathogenesis of these complications.
出处
《中国实验血液学杂志》
CAS
CSCD
2008年第4期838-842,共5页
Journal of Experimental Hematology
