异基因脐血干细胞移植后STR基因座位的表达分析

Analysis of STR Loci Expression after Allogeneic Cord Blood Hematopoietic Stem Cell Transplantation

本研究分析杜氏型肌营养不良(DMD)病人异基因脐带血干细胞移植后STR基因座位的表达。利用PCR-SSO方法检测患者、供者HLA-A、B、DR位点,并利用STR-PCR方法检测术后各个器官的STR基-因位点的表达情况。结果表明:患者与供者在HLA中、低分辨情况下A、B、DR位点全相合,患者的胸骨骨髓显示为供者独立植入,脾、左上肺、前臂、肌舌、左肝、胃、右颞叶、膈肌、右支气管、左心室、右肾均为嵌合状态。结论:造血干细胞移植术后,供者的基因可在实体器官表达,形成嵌合状态。

The purpose of this study was to analyze the STR loci expression after allergenic cord hematopoietic stem cell transplantation in patient with Ducennes muscular dystropy （DMD） patient. PCR-SSO was used to identify the HLA antigens and alleles, STR-PCR was used to detect the chimera status. Quantity analysis of donor chimeras was performed by multiplex PCR amplification of STR marker and capillary electrophoresis with fluorescence detection. The results showed that patient appear to be HLA identical to the donor cord blood at the tested level. Persistent full donor chimerism was found in breast bone marrow. The patient with stable MC （ DC 〈5% ） had a probability of long term survival with molecular remission MC status appeared in forearm muscle, tongue, liver, spleen, stomach, fight temporal lobe, diaphragmatic muscle, bronchus, left ventricle and fight kidney. In conclusion, the donor gene can express in parenchymatoas organs, the donor chimerism was detected in breast bone marrow and some other organs.