广东地区CML患者中HLA-A,B,DRB1的表达与分析

Expression and Analysis of HLA-A,B and DRB1 Genes in Patients with Chronic Myelogenous Leukemia in Guangdong Area

为了调查慢性髓系白血病(CML)患者中HLA-A、B、DRB1基因多态性的表达,探讨HLA与CML之间的可能关联,采用DNA扩增基础上的反向序列特异性寡核苷酸杂交(PCR-RSSO)技术,对广东地区293例CML患者和随机同期采集的406名汉族健康献血者的HLA-A、B、DRB1位点进行基因多态性分型,并对2组间基因频率进行了比对分析。结果显示:CML组HLA-A*24基因频率为15.53%,显著低于对照组22.09%(RR=0.63,p=0.005),HLA-B*13基因频率为10.41%,与对照组6.74%相比则显著增高(RR=1.68,p=0.016),HLA-DRB1*14基因频率为7.51%,与对照组11.89%相比显著降低(RR=0.58,p=0.008)。结论:HLA-A*24、HLA-DRB1*14在广东地区CML患者中表达较正常人明显减低,HLA-B*13在广东地区CML患者中表达较正常人明显升高。HLA-A*24、HLA-DRB1*14是否为广东地区CML患者的保护性基因标记及HLA-B*13是否为其易感性基因标记尚需进一步研究明确。

To study the gene polymorphism of HLA-A, B, DRB1 alleles in patients with chronic myelogenous leukemia and to explore the correlation of HLA with chronic myelogenous leukemia, the polymerase chain reaction- reverse sequence specific oligonucleotide （PCR-RSSO） was used to analyze the polymorphism of HLA-A, B, DRB1 alleles of 293 CML Patients and 406 randomized and synchronous blood donors （ healthy and unrelated with patients ） from Guangdong Han population. The results indicate that the gene frequency of HLA-A ＊ 24 in CML group was 15.53% lower than that of control group （22.09%, RR = 0. 63 ,p = 0. 005 ） ; the gene frequency of HLA-B ＊ 13 in CML group was 10.41% higher than that of control group （6.74% , RR = 1.68 ,p = 0.016）. The gene frequency of HLA- DRB1 ＊ 14 in CML group was 7.51% lower than that of control group （ 11.89%, RR = 0. 58, p = 0.008）. The differences were all statistically significant. It is concluded that the gene frequency of HLA-A ＊ 24,HLA- DRB1 ＊ 14 in CML patients is significantly lower than normal people in Guangdong. The gene frequency of HLA-B ＊ 13 in CML patients is significantly higher than normal people in Guangdong. Further study is needed to make sure whether HLA-A ＊ 24 and HLA- DRB1 ＊ 14 are protective gene markers for CML acquisition on Guangdong Chinese Han population and whether HLA-B ＊ 13 is a gene marker for CML susceptibility on this population.