野战运输对红细胞保存的影响

Effect of Highway Transportation on the Quality of Red Blood Cells

为了研究野战运输条件对几种血液成份的影响,为战时伤病员救治的血液保障提供依据,将红细胞悬液、少白细胞的红细胞悬液和洗涤红细胞经过模拟野战公路(三级公路)运输(震荡)4小时,然后在4℃条件下继续保存15天;取震荡前、后及保存15天的3个时间点的血样,分别进行上清游离血红蛋白、血常规和血生化分析。结果表明:红细胞悬液和少白细胞的红细胞悬液组游离血红蛋白、乳酸脱氢酶和钾离子浓度在震荡前、后及保存15天后的改变没有明显差异,而洗涤红细胞在震荡后上述三项指标显著增加,继续保存15天后,进一步增高。3个组中其它一些血液学指标没有明显的改变。结论:野战运输条件下(三级公路),采取合理的保温和减震措施后,运输4小时内红细胞悬液和少白细胞的红细胞悬液仍然可以继续保存15天并应用于临床,而洗涤红细胞则不能。

This study was purposed to investigate the effect of highway transportation on the quality of blood components so as to provide experimental basis to meet the needs of military operations. The transport condition was simulated by random vibration test. The red blood cells, leukocyte-reduced red blood cells, washed red blood cells were randomly vibrated （C Road） for 4 hours. Then, these blood components were stored in refrigerator for 15 days （4℃）. Six milliliters of blood were collected before vibration, after vibration, and at day 15 days of storage after vibration, respectively. The suspension was isolated. The free hemoglobin （FHb）, routine hematological parameters, and biochemical indexes were determined. The results showed that FHb, lactate dehydrogenase （LDH）, K^＋ of red blood cells and leukocyte-reduced red blood cells did not significantly change after vibration and storage. However, FHb, LDH and K^＋ of washed red blood cells increased significantly after simulated transportation （ p 〈0.05）. The levels of these parameters at day 15 of storage after vibration were also significantly higher than those after vibration （ p 〈 0. 01 ）. The changes of other hematological parameters were not significant in three blood components after vibration （C Road） and storage for 15 day. In conclusion, red blood cells and leukocyte-reduced red blood cells were qualified for clinic transfusion even after transportation within 4 hours for 15 day storage later, if they were kept in proper blood container and protected from damping. However, the washed red blood cells could not be used for clinic after similar transport in the military operations.