慢性荨麻疹患者转录因子T-bet、GA-TA-3表达失衡的研究

Unbalanced expression of T cell-specific transcription factors T-bet and GATA-3 in chronic urticaria

目的:探讨Th1和Th2细胞特异性转录因子T-bet和GATA-3在慢性荨麻疹患者免疫失衡中的作用和意义。方法:采用逆转录多聚合酶链反应(RT-PCR)方法分别检测33例慢性荨麻疹患者及20例健康对照者外周血单一核细胞(PBMC)中T-bet和GATA-3的表达水平。结果:慢性荨麻疹患者组PBMC中T-bet mRNA表达水平较对照组显著降低(P=0.006);而GATA-3 mRNA的水平显著高于正常人(P=0.000)。结论:T-bet/GATA-3的失衡参与了慢性荨麻疹的发病。

Objective： To investigate the role of T- bet and GATA - 3 in pathogenesis of chronic urticaria （CU）. Methods- T- bet mRNA and GATA - 3 mRNA in the peripheral blood mononuclear cells （PBMC） were detected by reverse transcription- polymerase chain reaction （RT- PCR） in 33 patients and 20 normal controls. Resuits： The expression of T- bet mRNA in the chronic urticaria patients was significantly decreased （P = 0.006）, while the level of GATA - 3 mRNA in the chronic urticaria patients was higher than that in controls （ P = 0.000）. Conclusion： Transcription factors T - bet/GATA - 3 may be involved in the immune imbalance in chronic urticaria.