摘要
目的:探讨Th1和Th2细胞特异性转录因子T-bet和GATA-3在慢性荨麻疹患者免疫失衡中的作用和意义。方法:采用逆转录多聚合酶链反应(RT-PCR)方法分别检测33例慢性荨麻疹患者及20例健康对照者外周血单一核细胞(PBMC)中T-bet和GATA-3的表达水平。结果:慢性荨麻疹患者组PBMC中T-bet mRNA表达水平较对照组显著降低(P=0.006);而GATA-3 mRNA的水平显著高于正常人(P=0.000)。结论:T-bet/GATA-3的失衡参与了慢性荨麻疹的发病。
Objective: To investigate the role of T- bet and GATA - 3 in pathogenesis of chronic urticaria (CU). Methods- T- bet mRNA and GATA - 3 mRNA in the peripheral blood mononuclear cells (PBMC) were detected by reverse transcription- polymerase chain reaction (RT- PCR) in 33 patients and 20 normal controls. Resuits: The expression of T- bet mRNA in the chronic urticaria patients was significantly decreased (P = 0.006), while the level of GATA - 3 mRNA in the chronic urticaria patients was higher than that in controls ( P = 0.000). Conclusion: Transcription factors T - bet/GATA - 3 may be involved in the immune imbalance in chronic urticaria.
出处
《中国麻风皮肤病杂志》
2008年第8期600-602,共3页
China Journal of Leprosy and Skin Diseases