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Correlation between expression and differentiation of endocan in colorectal cancer 被引量：11

Correlation between expression and differentiation of endocan in colorectal cancer
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摘要 AIM: To investigate the expression frequency of endocan in colorectal cancer and analyze the relationship between endocan expression and clinical parameters and to study the role of endocan in colorectal carcinogenesis. METHODS: Expression of endocan in 72 tumor tissue samples of colorectal cancer as well as in 27 normal mucous membrane tissue samples was analyzed using in situ hybridization, immunohistochemistry on tissue microarray, Western blot and reverse-transcript polymerase chain reaction (RT-PCR). RESULTS: The expression of endocan was higher in normal colon and rectum tissue samples than in cancerous tissue samples (mRNA = 92.6%, protein = 36%), and was lower in colorectal cancer tissue samples (mRNA = 70.4%, protein = 36.1%). No correlation was found between staining intensity and clinical parameters such as sex, age, tumor size andTNM stage. However, the expression of endocan was positively correlated with the tissue differentiation in colorectal cancer. CONCLUSION: The expression of endocan is down- regulated in colorectal cancer and is positively correlated with the tissue differentiation in colorectal cancer, suggesting that the expression of endocan is associated with development and differentiation of colorectal cancer. AIM： To investigate the expression frequency of endocan in colorectal cancer and analyze the relationship between endocan expression and clinical parameters and to study the role of endocan in colorectal carcinogenesis. METHODS： Expression of endocan in 72 tumor tissue samples of colorectal cancer as well as in 27 normal mucous membrane tissue samples was analyzed using in situ hybridization, immunohistochemistry on tissue microarray, Western blot and reverse-transcript polymerase chain reaction （RT-PCR）. RESULTS： The expression of endocan was higher in normal colon and rectum tissue samples than in cancerous tissue samples （mRNA = 92.6%, protein = 36%）, and was lower in colorectal cancer tissue samples （mRNA = 70.4%, protein = 36.1%）. No correlation was found between staining intensity and clinical parameters such as sex, age, tumor size and TNM stage. However, the expression of endocan was positively correlated with the tissue differentiation in colorectal cancer.CONCLUSION： The expression of endocan is downregulated in colorectal cancer and is positively correlated with the tissue differentiation in colorectal cancer, suggesting that the expression of endocan is associated with development and differentiation of colorectal cancer.
作者 Li Zuo Su-Mei Zhang Ruo-Lei Hu Hua-Qing Zhu Qing Zhou Shu-Yu Gui Qiang Wu Yuan Wang
机构地区 Laboratory of Molecular Biology and Department of Biochemistry Department of Respiratory Medicine Department of Pathology
出处 《World Journal of Gastroenterology》 SCIE CAS CSCD 2008年第28期4562-4568,共7页 世界胃肠病学杂志（英文版）
基金 Natural Science Foundation of Anhui Province, No. 050430705 National Natural Science Foundation of China, No. 30570750 Grant from Ministry of Education for Excellent Young Teachers in Anhui Medical University (kj002)
关键词 Endocan Colorectal cancer DIFFERENTIATION EXPRESSION In situ hybridization 结肠直肠癌 细胞分化 基因表达 杂合化
分类号 R735.3 [医药卫生—肿瘤]
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参考文献13

  • 1卜丽佳,左莉,任传利,张素梅,周青,桂淑玉,汪渊.LPS对人脐静脉内皮细胞增殖和ESM-1表达的影响[J].安徽医科大学学报,2005,40(1):7-9. 被引量：1
  • 2熊江霞,吴正升,吴强,周青,桂淑玉,汪渊.内皮细胞特异性分子1在肾癌组织的表达[J].中国肿瘤,2004,13(5):309-312. 被引量：8
  • 3熊江霞,吴强,周青,吴正升,桂淑玉,汪渊.肾癌组织中内皮细胞特异性分子-1 mRNA水平及其蛋白表达的关系[J].临床与实验病理学杂志,2004,20(2):187-190. 被引量：4
  • 4Ikeda K,Monden T,Kanoh T,Tsujie M,Izawa H,Haba A,Ohnishi T,Sekimoto M,Tomita N,Shiozaki H,Monden M.Extraction and analysis of diagnostically useful proteins from formalin-fixed, paraffin-embedded tissue sections[].Journal of Histochemistry and Cytochemistry.1998
  • 5Chung JY,Braunschweig T,Baibakov G,Galperin M,Ramesh A,Skacel M,Gannot G,Knezevic V,Hewitt SM.Transfer and multiplex immunoblotting of a paraffin embedded tissue[].Proteomics.2006
  • 6Timar J,Lapis K,Dudas J,Sebestyen A,Kopper L,Kovalszky I.Proteoglycans and tumor progression: Janus- faced molecules with contradictory functions in cancer[].Seminars in Cancer Biology.2002
  • 7Scherpereel A,Depontieu F,Grigoriu B,Cavestri B,Tsicopoulos A,Gentina T,Jourdain M,Pugin J,Tonnel AB,Lassalle P.Endocan, a new endothelial marker in human sepsis[].Critical Care Medicine.2006
  • 8Abid MR,Yi X,Yano K,Shih SC,Aird WC.Vascular endocan is preferentially expressed in tumor endothelium[].Microvascular Research.2006
  • 9Sarrazin S,Adam E,Lyon M,Depontieu F,Motte V,Landolfi C,Lortat-Jacob H,Bechard D,Lassalle P,Delehedde M.Endocan or endothelial cell specific molecule-1 (ESM-1): a potential novel endothelial cell marker and a new target for cancer therapy[].Biochimica et Biophysica Acta.2006
  • 10Seidel C,Borset M,Hjertner O,Cao D,Abildgaard N,Hjorth-Hansen H,Sanderson RD,Waage A,Sundan A.High levels of soluble syndecan-1 in myeloma-derived bone marrow: modulation of hepatocyte growth factor activity[].Blood.2000

二级参考文献17

  • 1Bosari S,Lee AKC,Delellis RA,et al. Microvessel quantitation and prognosis in invasive breast carcinoma[J].Human Pathol, 1992,23(7):755-761.
  • 2Bechard D,Scherpereel A, Hammad H,et al. Human endothelial-cell specific molecule-1 binds directly to the integrin CD l 1 a/CD 18(LFA- 1)and blocks binding to intercellular adhesion molecule-1 [J].J Immunol,2001,167 (6):3099-3106.
  • 3Aitkenhead M,Wang SJ,Nakatsu MN,et al.Identification of endothelial cell genes expressed in an in vitro model of angiogenesis:induction of ESM-1, (beta)ig-h3 ,and NrCAM[J].Microvasc Res, 2002,63(2): 159-171.
  • 4Bechard D,Meignin V,Scherpereel A,et al. Characterization of the secreted form of endothelial-cell-specific molecule 1 by specific monoclonal antibodies [J]. J Vasc Res,2000,37(5):417-425.
  • 5Lassalla P, Molet S, Janin A, et al.ESM-1 is a novel human endothelial cell-specific molecule expressed in lung and regulated by cytokines [J]. J Biol Chem, 1996,271(34):20458-20464.
  • 6Tsai JC, Zhang J,Voland C, et al. Cloning and characterization of the human lung endothelial-cell-specific molecule-1 promoter[J]. J Vasc Res, 2002,39(2):148-159.
  • 7Fuhrman SA,Laskylc LC,Limas C.Prognostic significance of morphologic parameters in renal cell carcinoma[J].Am J Surg Pathol, 1982,6(7):655-663.
  • 8Weinder N,Folkman J,Pozza F,et al. Tumor angiogenesis:a new significant and indenpdent prognostic indicator in early-stage breast carcinoma [J].J Natl Cancer Inst, 1992,84:1875-1887.
  • 9Lassalla P, Molet S, Janin A, etal. ESM-1 is a novel human endothelial cell-specific molecule expressed in lung and regulated by cytokines. J Biol Chem, 1996,271(34):20458～64.
  • 10Tsai JC, Zhang J, Voland C, etal. Cloning and characterization of the human lung endothelial-cell-specific molecule-1 promoter.J Vasc Res, 2002,39(2):148～59.

共引文献10

同被引文献29

  • 1张文健,吴练秋,叶丽亚,娄晋宁.肿瘤血管内皮细胞的免疫学特性[J].癌症,2005,24(5):634-638. 被引量：5
  • 2董蕾,吴强,胡向阳,丁向东,汪渊,杨枫,左莉.涎腺腺样囊性癌组织中ESM-1 mRNA及其蛋白表达的关系[J].山东医药,2006,46(19):17-19. 被引量：2
  • 3Depontieu F, Grigoriu BD, Scherpereel A, et al. Loss of endocan tu- morigenic properties after alternative splicing of exon 2 [ J ]. BMC Cancer, 2008, 8: 14.
  • 4Sarrazin S, Bechard D, Lassalle P, et al. Purification and charac- terization of endocan ( endothelial cell-specific molecule-1 ) , a cir- culating proteoglycan involved in tumour progression and inflamma- tory diseases[ J]. Int J Exp Pathol, 2004, 85 (4) :A73.
  • 5Aitkenhead M, Wang SJ, Nakatsu MN, et al. Identification of en- dothelial cell genes expressed in an in vitro model of angiogenesis : induction of ESM-1, (beta) ig-h3, and NrCAM [ J]. Microvasc Res, 2002, 63(2) : 159-171.
  • 6De Freitas Caires N, Barrier M, Sarrazin S, et al. Identification of cathepsin G in the generation of elastase-resistant fragment of vascular endocan: involvement in the regulation of LFA-I-de- pendent cascade[ J]. Crit Care, 2009, 13 (Suppl 4) : P16.
  • 7Shin JW, Huggenberger R, Detmar M. Transcriptional profiling of VEGFA and VEGF-C target genes in lymphatic endothelium reveals endothelial-specific molecule-1 as a novel mediator of lymphangiogenesis [ J ]. Blood, 2008, 112 ( 6 ) : 2318-2326.
  • 8Aird WC. Phenotypic heterogeneity of the endothelium: struc- ture, function, and mechanisms [ J ]. Circ Res, 2007, 100 : 158-173.
  • 9van't Veer LJ, Dai H, van de Vijver M J, et al. Gene expression profiling predicts clinical outcome of breast cancer [ J ]. Nature, 2002, 415(6871 ) :530-536.
  • 10刘从云,吴正升,张晴,丁向东,左莉,汪渊,张瑰红,吴强.乳腺癌组织中ESM-1的表达及其预后意义[J].安徽医科大学学报,2008,43(4):391-395. 被引量：5

引证文献11

二级引证文献21

World Journal of Gastroenterology
2008年 第28期

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