RhoC基因在胃癌中的表达及其意义

The expression of RhoC mRNA in gastric carcinoma.

目的 究胃癌与癌旁胃黏膜中RhoC mRNA的表达情况及其与胃癌主要临床病理特征的关系。方法 采用半定量RT-PCR方法检测23例新鲜胃癌组织和相应的癌旁胃黏膜组织中RhoC mRNA的表达。结果 胃癌组织中RhoC mRNA的表达水平高于相应的癌旁组织，其吸光度比值分别为1．40±0．23和0．36±0．15（P〈0．01）；同时RhoC mRNA在胃癌组织中的表达水平与胃癌浸润深度、TNM分期和有无淋巴结转移有相关性（均P〈0．01）。结论 RhoC mRNA在胃癌组织中过表达可能与胃癌的发生发展密切相关，有望成为胃癌的早期诊断指标。

Objective To investigate the expression of RhoC mRNA in the gastric carcinoma （GC） and paracarcinoma gastric mucosa tissues （PGMT） and their relationship with clinical pathological features. Methods RhoC mRNA was examined by semi-quantitative reverse transcription polymerase chain reaction （RT-PCR） in 23 cases of primary gastric carcinoma and the paracarcinoma gastric mucosa tissues. Results The opacity density of RhoC mRNA in 23 cases of GC was 1.40 ± 0.23 ,higher than that of PGMT （ 0.36 ±0.15 ） （ P 〈 0.01 ）. In addition,RhoC mRNA in gastric carcinoma tissues were positively related to invasion depth ,TNM stage and lymph node metastasis（ P 〈 0.01 ）, Conclusion The overexpression of RhoC mRNA in GC may be closely related to the carcinogenesis, development, invasion and metastasis of gastric carcinoma, which is a frefered biomarker for early diagnosis.