两种藻胆蛋白对人喉癌Hep-2细胞的光动力杀伤效果

Photodynamic Effect of Two Kinds of Phycobiliproteins on Human Laryngeal Cancer Cell Line Hep-2

从条斑紫菜中提取高纯度R-藻红蛋白(R-PE)和R-藻蓝蛋白(C-PC),采用MTT法测定主要研究了不同浓度(10、25、50和100μg/ml)R-PE和C-PC分别介导的光动力效应对人喉癌Hep-2细胞的生存率的影响。实验结果显示,两种藻胆蛋白的光动力作用对Hep-2细胞具有杀伤作用。在浓度为100μg/ml,照射剂量为50J/cm2的条件下,藻蓝蛋白对应的细胞存活率为64%,藻红蛋白对应的仅为57%;单用碘钨灯处理,Hep-2细胞的存活率达到86.9%;而单独使用这两种藻胆蛋白处理Hep-2细胞,培养24h后,低浓度藻胆蛋白(10、25μg/ml)对细胞的抑制效果不明显,高浓度对细胞生长具有一定的抑制效果,抑制率为68%。实验证明条斑紫菜R-藻红蛋白和C-藻蓝蛋白具有可开发人喉癌治疗光敏剂应用前景。

In this paper,high purified R-Phycoerythrin （R-PE）and C-Phycocyanin （C-PC）were extracted from Porphyra yezoensis, and they were applied as photosensitizer to study on inhibitory effect of photodynamic reaction on human laryngeal cancer cell line Hep-2. With MTT assay,effects of R-Phycoerythrin and C-Phycocyanin concentrations （10,25,50 and 100μg/ml）and illumination dose on cancer cell photodynamic therapy reaction with irradiation of iodine tungsten lamp were analyzed. The results showed that the photodynamic effects of these two phycobiliproteins on Hep-2 were remarkable. With 100μg/ml concentration of C-PC and R-PE and 50J/cm^2 illumination dose of iodine tungsten lamp,the survival rates of the cancer cells were 64% and 57%,respectively. The survival rates of the cancer cells only treated by illumination of iodine tungsten lamp was 86.9%. The inhibition rates of the cancer cells treated only with higher concentration of phycobiliproteins （R-PE,C-PC ≥50μg/ml）were about 68% after they cultured for 24hs. It was indicated that the R-Phycoerythrin and C-Phycocyanin extracted from Porphyra yezoensis would become a new ldnd of photosensitizer and could be applied to photodynamic therapy of cancer.