外界因子对条斑紫菜自由壳孢子囊枝形成和生长影响

Effect of External Factors on Formation and Growth of Free-living Conchosporangia in Porphyra yezoensis

主要研究了不同光照周期(8L:16d、12L:12d和16L:8d)、不同温度(15℃、20℃和25℃)、不同光照强度(29、42、57、72和86μmol m-2.s-1)等外界因子对条斑紫菜自由丝状藻丝切断诱导形成壳孢子囊枝影响,以期获得条斑紫菜壳自由孢子囊枝形成的最佳外界条件。结果表明,在25℃和57μmol m-2·s-1条件培养30d后,短日照(8L:16d)对壳孢子囊枝诱导形成效果最好,其壳孢子囊枝形成率高达92.5%,50d后几乎达到100%;在25℃和短日照条件培养30d后,光照强度为57μmol m-2·s-1时,壳孢子囊枝诱导形成率达到最高(92.2%),光照强度过高过低均不利壳孢子囊枝形成;在57μmol m-2·s-1和短日照条件下,当温度为25℃时,壳孢子囊枝形成率最高,形成率随温度下降而减少。在12L:12d光照周期、57μmol m-2·s-1光照强度和25℃温度条件下,自由壳孢子囊枝生长最快,其日平均相对生长率可达54.43%。以上研究为实现紫菜细胞工程育苗新技术-自由壳孢子囊枝育苗奠定了坚实基础。

In this paper,effects of external factors of different photoperiod （8L： 16d,12L： 12d and 16L： 8d）, temperature （ 15℃, 20℃, 25℃ ）and light intensity （29,42,57,72 and 86 μmol m^-2·s^-1）on the free-living conchosporangia formation from free-living conchocelis filament cuttings and growth in Porphyra yezoensis HB,line were studied. When free-living conchocelis were cultured at 25℃ and 57μmol m^-2·s^-1 for 30 days,the formation rates of free-living conchosporangia in short photoperiod （SL： 16d）groups were the highest,as high as 92.5%. When they were cultured for more than 50 days,formation rates reached up to 100%. Cultured at 57μmol m^-2·s^-1 and 8L：16d for 30 days,the formation rates of free-living conchosporangia in 57μmol m^-2·s^-1 light intensity groups,were 92.2%. If light intensity was too high or too low,the conchosporangia formation would be inhibited. With 57μmol m^-2·s^-1 and 8L：16d,formation rates of free-living conchosporangia at 25.℃ groups was the highest,and free-living conchosporangia rate would decrease with temperatures decreasing. At 57μmol m^-2·s^-1,12 L：12d and 25℃ ,daily relative growth rate of free-living conchosporangia reached as high as 54.43%. The study data would lay an important foundation for further development of new breeding technology used for free-living conchosporangia.