期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

一种高效可直接用于PCR扩增的不吸水链霉菌基因组DNA的提取方法 被引量：2

A Hi-Effect Extraction Method Used Directly for PCR to Genome DNA of Streptmyces Ahygroscopicus
下载PDF
导出
摘要 通过对提取不吸水链霉菌基因组DNA的冻融法、微波法和溶菌酶破壁法等几种方法进行对比、分析,得出结论:溶菌酶破壁法所提取的不吸水链霉菌基因组DNA可直接用于PCR扩增。这为今后不吸水链霉菌相关基因的实验研究提供了可靠的理论依据。 The extraction of Genome DNA of Streptmyces Ahygroscopicus were compared and analyzed through the methods of freeze-thaw,microwave and break-shell of lysozyme. The results showed that the Genome DNA of Streptmyces Ahygroscopicus extraction through the method of the break-shell of lysozyme can be used directly for PCR. The study gave a theoretical basis for the future lab research on relative gene of Streptmyces Ahygroseopicys.
作者 吴红艳 陈飞 桓明辉 关艳丽 郭玲玲
机构地区 辽宁省微生物科学研究院
出处 《生物技术通报》 CAS CSCD 2008年第4期195-197,202,共4页 Biotechnology Bulletin
关键词 不吸水链霉菌 基因组DNA 提取 PCR扩增 Streptmyces ahygroscopicus Genome DNA Extraction PCR
分类号 S476 [农业科学—农业昆虫与害虫防治] Q78 [生物学—分子生物学]
  • 相关文献

参考文献4

二级参考文献21

  • 1日本放线菌学会编.放线菌の分类と同定[M].,2001.83，88.
  • 2J萨姆布鲁克 EF弗里奇 T曼尼阿蒂斯.《分子克隆》(第二版)[M].北京：科学出版社,1996..
  • 3萨姆布鲁克J. 费里奇E.F. 曼尼阿蒂斯T. 主编 金冬雁 黎孟枫主译.分子克隆实验指南(第二版)[M].科学出版社,中国,北京,1999.pp.343-362.
  • 4Orsini M, Romano-Spiea V. Lett Appl Microbiol, 2001, 33:17～20.
  • 5Hopwood D H, Bibb M J, Chater K F, et al. Genetic Manipulation of Streptomyces A Laboratory manual. In Preparation of chromosomal, plasmid and phage DNA. Norwich: F. Crowe & Sons Ltd. 1985, 79～80.
  • 6Kinya Fujishiro, Toshio Ota, Mamoru Hasegawa, Kazuo Yamaguchi,et al. Isolation and Identification of the Gene of Cholesterol Oxidase from Brevibactrium Sterolicum ATCC 21387, a Widely Used Enzyme in Clinical Analysis[J ]. Biochemical and Biophysical Research Communications,1990,172(2) :721-727.
  • 7Kajal Bose Ghoshroy, Weiqun Zhu, Nicole S. Sampson. Investigation of Membrane Disruption in the Reaction Catalyzed by Cholesterol Oxidase[J ]. Biochemistry, 1997,36: 6133-6140.
  • 8J T Greenplate, N B Duck, J C Pershing&J P Purcell. Cholmterol Oxidase:an O?static and Larvicidal Agent Active Against the Cotton Boll Weevil ,Anthonomus Grandis[J]. Entomologia Experimentalis et Applicata, 1995,74:253-258.
  • 9JFSúS NAVAS, BRUNO GONZáLEZ-ZORN, NéSTOR LADRóN, PATRICIA GARRIDO, JOSé A. VáZQUEZBOLAND. Identification and Mutagenesis by Allelic Exchang of choE,Encoding a Cholesterol Oxidase from the Intracellular Pathogen Rhtxiococcus equi[J]. Journal of Bacterology, 9.001,183(16):4796-4805.
  • 10George A Somkuti,Daniel K y etc. Transfer and Expression of a Streptomyces Cholesterol Oxidase Gene in Streptococcus thermophilus[J]. Biotechnology and Applied Biochemistry, 1991,13 : 238- 245.

共引文献295

同被引文献13

  • 1刘晓永,王强,胡永金.用微珠涡流法破壁酵母细胞[J].吉首大学学报（自然科学版）,2006,27(6):110-113. 被引量：9
  • 2Freimund S,Sauter M, Kappeli O,et al. A new non-degrading isolation process for 1,3-13-D-glucan of high purity from Baker' s Yeast Saccharomyces Cerevisiae. Carbohydrate Polymers, 2003, 54 : 159-171.
  • 3萨姆布鲁J,拉塞DW.分子克隆实验指南(精编版)[M].北京:化学工业出版社,2008,150-151.
  • 4Griffiths LJ,Yim M,Doffmam SR,et al. Comparison of DNA extraction methods for Aspergillus fumigatus using real-time PCR. J Med Microbiol, 2006,55 ( 9 ) : 1187-1191.
  • 5Mohamed MA, Koichi M,Tsuyoshi Y, et al. Direct colony PCR of several medically important fungi using Ampdirect plus. Jpn J Infect Dis ,2009,62 : 164-167.
  • 6Mirhendi H, Diba K, Rezaei A. Colony-PCR is a rapid and sensitive method for DNA amplification in yeasts. Iranian J Publ Health, 2007,36( 1 ) :40-44.
  • 7Shirling EB,Gottlieb D, Methods for characterization of Streptomyces species. Intern J Syst Bact,1966,16(3) :313-340.
  • 8董德鑫 胡永兰 程国芬.不吸水链霉菌的一个新亚种[J].微生物学报,1988,28(1):85-87.
  • 9王志博,潘军,张永根,娄丽平.用酶法对啤酒酵母细胞破壁优化条件的研究[J].东北农业大学学报,2008,39(3):76-79. 被引量：17
  • 10郭卫芸,杜冰,袁根良,杨公明.反复冻融法破壁啤酒废酵母的研究[J].酿酒科技,2009(3):103-105. 被引量：32

引证文献2

二级引证文献14

生物技术通报
2008年 第4期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部