RP-HPLC同时测定清热明目茶中3个成分的含量

Determination of three compounds in Qingre Mingmu Tea by RP-HPLC

目的：建立同时测定清热明目茶（决明子、菊花等）中大黄素、大黄酚、大黄素甲醚含量的反相高效液相色谱法。方法：以DiamonsilC18（250mm×4．6mm，5μm）为固定相，乙腈-0．1％磷酸为流动相，在0到20min，乙腈量从70％上升到85％，检测波长为254nm，流速1mL／min。结果：3种成分分离良好，大黄素，大黄酚，大黄素甲醚进样量分别在范围5.335～213．4ng，9．075～363．0ng和4．688—187．5ng线性关系良好，3种化合物的加样回收率分别为98．9％，104．0％和101．4％。结论：本法简单、快捷、适合于测定清热明目茶中大黄素、大黄酚、大黄素甲醚的含量。

AIM： To develop a RP-HPLC method for simultaneously determining three major compounds in Qingre Mingmu Tea （Semen Cassiae, Flos crysanthemi, etc） , namely emodin, chrysophanol and physcion with UV detection wavelength at 254 nm. METHODS： The column was Diamonsil Cls （250 mm×4.6 mm i. d, 5μm）. The separation was carried out with a gradient program. a flow rate of 1.0 mL/min. RESULTS： The standard 0.999 8 ） for emodin, 9. 075-363.0 ng （ r = 0.999 8 ） The mobile phase was acetonitrile-0.1% phosphoric acid at curve was rectilinear in the range of 5. 335-213, 4 ng（ r = for chrysophanol, and 4.688-187.5 ng（ r = 1. 000 0） for physcion, respectively. The recoveries of the markers listed above were 96.0%, 106.5% and 98.1%, respectively. CONCLUSION： The method is simple, rapid and suitable for the determination of three compounds in Qingre Mingmu Tea.