摘要
目的观察沙立度胺对人乳腺癌细胞系MCF-7、MDA-MB-231细胞血管内皮生长因子（VEGF）表达的抑制作用。方法应用半定量逆转录-聚合酶链反应（RT—PCR）技术，分别观察沙立度胺对MCF-7和MDA-MB-231细胞VEGF表达的抑制作用。结果沙立度胺在50μg／L明显抑制两株细胞VEGF mRNA的表达，但浓度增高到100μg／L抑制VEGF mRNA表达的作用反而减弱。结论沙立度胺在一定浓度范围内能抑制乳腺癌细胞VEGF mRNA的表达。
Objective To assess the effects of thalidomide on VEGF mRNA level in human breast cancer cell lines. Methods Breast cancer cells were treated with thalidomide for 24-72h, RT-PCR was uced to detect the level of VEGF mRNA expression. Results When the concentration of drug is 50μg/L,thalidomide strongly inhibited the level of VEGF mRNA expression. Conclusion Within certain concentration range of thalidomide can inhibit the level of VEGF mRNA expression in breast cancer cell.
出处
《中国基层医药》
CAS
2008年第7期1057-1058,1233,共3页
Chinese Journal of Primary Medicine and Pharmacy
基金
河北省科技攻关项目（062761401）
关键词
乳腺肿瘤
沙立度胺
血管内皮生长因子
细胞系
Breast neoplasm
Thalidomide
Vascular endothelial growth factor
Cell lines
