姜黄素对UVB辐射诱导HaCaT细胞凋亡的抑制作用

Inhibitory effects of curcumin on HaCaT cell apoptosis induced by UVB irradiation

目的:建立中波紫外线(UVB)对体外培养的永生化人角质形成细胞(HaCaT细胞)辐射损伤病理模型,探讨姜黄素(Cur)对其细胞辐射损伤的保护作用。方法:以10、20、30、40和50m J/cm2的UVB辐照其细胞,分别在辐照后6、12、18、24、48和72h,用MTT和流式细胞仪检测细胞活性及其凋亡的变化,建立辐照损伤病理模型;用30m J/cm2的剂量辐照其细胞后,立即分别给予0.625、1.25、2.5和5μg/mL的Cur处理,在处理18h后检测其细胞凋亡。结果:HaCaT细胞经UVB辐照后,以剂量和时间依赖方式抑制其细胞存活,且主要由于细胞凋亡引起;Cur以浓度依赖方式抑制由UVB所致的细胞凋亡。结论:Cur能抑制UVB辐射诱导的HaCaT细胞凋亡,对UVB辐射损伤HaCaT细胞具有保护作用,其作用机制可能与Cur清除体内自由基而增强细胞的抗氧化能力有关。

Objective ： To investigate whether Cur could inhibit the apoptosis of immortalized human keratinocyte HaCaT cells induced by ultraviolet B （UVB） in vitro. Methods ：Cultured HaCaT cells were irradiated with UVB of 10, 20, 30, 40 and 50 mJ/cm^2. Cellular viability and apoptosis were detected by MTT and flow cytomytry, respectively, 6, 12, 18, 24, 48 and 72h after irradiation in order to determined injury model. HaCaT cells were treated with Cur of 0. 625, 1.25, 2.5 and 5μg/ mL, respectively, after irradiation with 30 mJ/cm^2 and cell apoptosis was detected 18 h after the irradiation. Results： UVB irradiation inhibited the viability of HaCaT cells in a dose - and time - dependent pattern. The cell viability decreased with the increase of irradiation doses and caused mainly by apoptosis ; Cur inhibited the apoptosis of HaCaT cells induced by UVB irradiation in a dose - dependent pattern. Conclusion ： Cur could protect HaCaT cells through inhibiting the apoptosis induced by UVB irradiation. The results suggest that Cur may have the effect of discarding free radicals in order to enhance oxidative damage.