异氟醚全凭吸入诱导下经口气管插管法在大鼠模型制作中的应用

Aapplication of Oral Trachea Intubation after Induction with Isoflurane Inhalation in Rats

目的:评价异氟醚全凭诱导麻醉下大鼠经口气管插管方法的可行性。方法:SD雌性大鼠20只随机分成两组各10只,A组采用气管切开法插管,B组采用经口气管插管法,比较两种方法的优缺点。结果:A组大鼠均能一次性插管成功,在缝合大鼠气管切口时,有1只大鼠因麻醉深度控制不当(过浅),缝合过程中诱发喉痉挛而窒息死亡。有2只大鼠在拔除导管后,麻醉较深而出现呼吸抑制,紫绀状。A组大鼠清醒后均出现嘶叫,对外界声响异常警觉、敏感,8h后仍不能正常进食、饮水。B组大鼠中,有8只大鼠为一次性插管成功,2只需要重新插管,停止吸入异氟醚后在2～3min内清醒,无1例出现紫绀或死亡。B组大鼠拔除导管后,均未出现嘶叫,1h后精神状态佳,2h后活动自如,自由饮水。结论:采用异氟醚全凭诱导麻醉下经口气管插管的方法是一种简便、无创性的气管插管方法,麻醉的深度、稳定性及持续时间易于掌握;不作气管切开,对动物损伤小,非实验因素的干扰小。

Objective： To evaluate the method of oral trachea intubation with the isoflurane induction in rats. Methods： Twenty female SD rats were randomly divided into 2 groups： group A and group B. Incision of trachea intubation was used in group A, and oral trachea intubation was used in group B. To compare the advantage and disadvantage with the two groups. Results： All rats were successfully intubated at one time in group A. One rat was suffocated to death from laryngospasm during saturation because of the insufficient anesthesia. Two rats had respiratory depression and cyanosis because of deep anesthesia after extubation. Rats in group A all had vocalization after consciousness, and especially alert to outside noise. Eight rats were successfully intubated at one time in group B, and two rats had the second intubation. All rats woke up in 2-3 minutes after the cessation of isoflurane inhalation. Rats of group B all had no vocalization after extubation. The mental status was good after one hour , and the rats acted as normal after two hours. Conclusion：The application of oral trachea intubation after induction of isoflurane is convenient and non invasive. The advantage of this method is that it is noninvasive and anesthesia is easily controlled and its interference factors was decraesd.