长期吸入糖皮质激素对支气管哮喘患儿血清中IL-6,IL-8和TNF-α水平的影响

Effect of long-term inhaled glucocoticoids on the levels of IL-6,IL-8 and TNF-α in bronchialasthamtic children

目的观察支气管哮喘患儿血清中IL-6、IL-8和TNF-α水平的变化并探讨支气管哮喘患儿在长期吸入糖皮质激素后对血清中IL-6、IL-8和TNF-α的影响。方法采用酶联免疫吸附(ELISA)的方法,分别检测62例哮喘患儿采用吸入糖皮质激素治疗前,治疗3、6、12个月后及30例正常对照儿童血清中IL-6、IL-8和TNF-α水平。结果哮喘患儿血清中IL-6、IL-8和TNF-α水平显著高于正常对照组。吸入糖皮质激素治疗3个月后哮喘患儿血清中IL-6、IL-8和TNF-α浓度与治疗前比较下降(P值均<0.05);治疗6个月和12个月后哮喘患儿血清中IL-6、IL-8和TNF-α浓度显著低于治疗前(P值均<0.01),且治疗12个月后各个炎症指标与对照组相比无显著性差异(P>0.05)。结论长期吸入糖皮质激素是治疗儿童支气管哮喘的重要手段,其治疗作用与下调血清中IL-6、IL-8和TNF-α水平有关。

Objective To observe the effect of long-term inhaled glucocoticoids on the levels of IL-6, IL-8 and TNF-α in bronchialasthamtic children. Methods Enzyme-linked Immunosorbent Assay （ELISA） was used to detect the serum IL-6, IL-8 and TNF-α levels in 30 healthy children （as a normal control group） and 62 asthmatic children （untreated and post-treated for 3, 6 and 12 months） Results The levels of IL-6, IL-8 and TNF-α in the serum were significantly increased in children with asthma and the difference was significant compared with that in the normal control group. The levels of IL-6, IL-8 and TNF-α in ehildren with asthma were decreased gradually after inhaling glucocoticoids for 3 months （P〈0. 05） 3 And the treatment of the inhaled glucocoticoids for 6 and 12 months can attenuate the elevation of IL-6, IL-8 and TNF-α as compared with that before the treament. Conclusion Long-term inhaled glucocoticoid is an important method for asthma, and the effect is related to the decrease of the levels of IL-6, IL-8 and TNF-α.