响应面法优化超声提取杜仲雄花中黄酮类化合物的工艺参数

Optimization of Ultrasound-assisted Extraction Process Parameters of Flavonoids from Eucommia ulmoides Staminate Flower by Response Surface Methodology

在单因素试验的基础上,对乙醇浓度、提取时间和超声功率三因素进行二次通用旋转组合试验设计并通过响应面分析,优化杜仲雄花中黄铜类化合物的超声波提取工艺。结果表明:超声辅助提取杜仲雄花中黄酮类化合物的最佳工艺参数为乙醇浓度60%、提取时间34min、超声功率320W。在此条件下,黄酮类化合物的实际提取率为13.13mg/g,与预测值基本一致。在三个因素中,乙醇浓度对提取率影响最大,其次是超声功率,提取时间对黄酮类化合物的提取率影响较小,并且三因素之间无交互作用。

On the basis of single-factor test, the ultrasound-assisted extraction process parameters of flavonoids from Eucommia ulmoides staminate flower were optimized by the quadratic general spinning design on ethanol concentration, extraction time and ultrasonic power. The results showed that the optimal parameters are as follows： the ethanol concentration 60%, the extraction time 34 min and the ultrasonic power 320 W. Under these conditions, the actual extraction rate of the flavonoids is 13.13 mg/g, consistent with the prediction value. Among the three factors, the alcohol concentration most significantly affects on the extraction rate, followed by the ultrasonic power and the extraction time, and no interaction exists among them.