微波辅助提取刺槐种子中黄酮类化合物的工艺参数优化

Optimization of Microwave-assisted Extraction Process Parameters of Flavonoids from Robinia persudoscacia Seeds

采用二次通用旋转组合设计研究了刺槐种子中黄酮类化合物的提取工艺。结果表明:四个因素对刺槐种子中黄酮类化合物提取率影响大小的顺序依次为:乙醇浓度>功率>时间>料液比。其中乙醇浓度、微波功率及二者的交互项对刺槐种子中黄酮类化合物的提取率有极显著影响;微波时间有显著影响;料液比无显著影响。最佳工艺参数为:乙醇浓度60%、微波功率800W、萃取时间40s、料液比1:30。在此条件下,刺槐种子中黄酮类化合物的理论提取率为31.99mg/g,验证值31.47mg/g,二者基本一致。

The extraction technology of flavonoids from Robiniapersudoscacia seeds was studied using quadratic general spinning design. The results showed that the extraction rate of flavonoids is mostly influenced by ethanol concentration, followed by microwave power, time and material to liquid ratio. The ethanol concentration and microwave power and the interaction between them have most significant effects on the extraction rate of flavonoids, and the effect of the microwave time takes second place, but the material to liquid ratio has no significant effect. The optimum process parameters are ethanol concentration 60%, microwave power 800 W, extraction time 40 s and material to liquid ratio 1：30. Under these conditions, the theory extraction rate of flavonoids from Robinia persudoscacia seeds is 31.99 mg/g. The result of verification experiment is 31.47 mg/g, close to that.