硒化复合L-氨基酸制备工艺研究

Study on Preparation Technology of Selenide Compound L-Amino Acid

以豆粕为原料,利用酶水解工艺制备复合L-氨基酸,将复合L-氨基酸与亚硒酸钠螯合制备硒化复合L-氨基酸。结果表明,豆粕酶解的最佳工艺条件为:固液比1:25、pH8.5、加酶量10%胰蛋白酶与8%木瓜蛋白酶、酶解时间均为4h,所得豆粕水解率为39.61%。复合L-氨基酸与亚硒酸钠螯合的最佳工艺条件为:温度80℃、pH9.0、摩尔配比2:1、时间2h。此条件下螯合率为43.28%,经红外光谱分析复合L-氨基酸与亚硒酸钠之间存在螯合作用。

This study aimed at the chelation technology of sodium selenite and compound L-amino acid from soybean meal by enzymatic hydrolysis. The results showed that optimum enzymatic hydrolysis condition for compound L-amino acid are as follows： ratio of material to liquid 1：25 （W/V）, pH 8.5 and hydrolysis time 4 h of 10% （addition amount） trypsin and 8% （addition amount） papain respectively. Under these conditions the hydrolysis rate is 39.61%. The optimum chelation conditions for compound L-amino acid and sodium selenite are determined through orthogonal test as follows： chelation temperature 80 ℃, pH 9.0 and molar ratio between them 2：1. The chelation ratio is 43.28% under these conditions. Finally the chelation of complex L-amino acid and sodium selenite is confirmed by infrared spectroscopy.