辛格粒毛盘菌胞内多酚提取及其抗氧化性研究

Study on Intracellular Polyphenol Extraction from Lachnum singerianum and Its Antioxidant Activities

在单因素试验的基础上,进行正交试验,以最大吸收波长下乙醇提取液吸光度为指标,探讨辛格粒毛盘菌胞内多酚(YM-82多酚)的提取工艺。所得最佳提取条件为:乙醇浓度80%、液料比50:1、时间9h。对YM-82多酚的抗氧化性进行研究,结果表明:浓度为21μg/ml的YM-82多酚,其还原能力强于25mg/ml的VC对照;浓度为24.7μg/ml的YM-82多酚对DPPH·清除率达到33.08%,为30mg/ml VC对照的1.18倍;浓度为22μg/ml的YM-82多酚对·OH清除率达到63.8%,远高于50mg/ml VC(对照),显示出YM-82多酚具有很强的抗氧化能力。

On the basis of single factor test, the extraction process of polyphenol from filamentous fungi Lachnum singerianum YM-82 was researched by orthogonal test through the absorbance determined at the typical peak. The results showed that the optimal conditions are as follows： concentration of ethanol 80%, ratio of liquid to solid 50：1 and time 4 h. The antioxidant and free radical scavenging activities of polyphenol from L singerianum YM-82 were evaluated using hydroxyal radical, superoxide anion radical and 1,1-diphenyl-2- picrylhydrazyl （DPPH） in vitro model systems. The results showed that the polyphenol has strong antioxidant and free radical scavenging activities. The reducing power of the polyphenol of 21 μg/ml is stronger than that of 25 mg/ml VC. The clearance rate to DPPH · of the polyphenol is 33.08% at 24.7 μ g/ml concentration, 1.18 times of that of 30 mg/ml VC, and the clearance rate to hydroxyal radical of the polyphenol is 63.8% at 22 μg/mlconcentration, significantly higher than that of 50 mg/ml VC system.