巴戟天多糖的提取及纯化研究

Study on Extraction and Purification of Polysaccharides from Morinda officinalis How

采用纤维素酶水解方法提取巴戟天粗多糖,并通过离子交换和凝胶过滤层析对得到的粗多糖进行了分离纯化。正交试验得到最佳的酶处理条件为:纤维素酶用量8mg/g、pH5.0、水解温度40℃以及水解时间2h。采用阶段梯度洗脱方式,巴戟天多糖在DEAE-Sephrose CL-6B柱上被分为三个组分:MOPI-1、MOPI-2和MOPI-3。将离子交换层析得到的MOPI-2和MOPI-3组分进行Sephadex G-50凝胶过滤层析,由MOPI-3可得到均一组分MOPI-3a,而MOPI-2被分为MOPI-2a和MOPI-2b两个组分。

The polysaccharides were extracted from Morinda officinalis How by pretreatment of cellulose, and were further purified by ion exchange and gel filtration chromatography. The optimal conditions for pretreatment of cellulose are determined by orthogonal test as follows： amount of ceUulase 8 mg/g, pH 5.0, hydrolysis temperature 40 ℃ and hydrolysis time 2 h. Three fractions, namely MOPI-1, MOPI-2 and MOPI-3 are obtained using the step gradient elution manner on DEAE-Sephrose CL- 6B column, and then the MOPI-2 and MOPI-3 fractions were purified by the gel filtration chromatography with Sephadex G- 50. An uniform fraction named as MOPI-3a is obtained from MOPI-3, while two fractions named as MOPI-2a and MOPI-2b are obtained from MOPI-2.