β-葡萄糖苷酶产生菌摇瓶发酵条件的研究

Study on Conditions Optimization of Shake Flask Fermentation for Producing β-glycosidase

本实验对产β-葡萄糖苷酶融菌株的摇瓶发酵条件进行了研究。采用正交交互试验对融合菌株发酵生产β-葡萄糖苷酶的摇瓶发酵条件进行优化。结果表明,最优摇瓶发酵条件:装液量20ml/250ml,接种量12%,发酵温度27℃,摇床转速180r/min,发酵时间7d。此条件下,β-葡萄糖苷酶活力为5.312U/ml。

In this study, the shake flask fermentation condition for producing β -glycosidase by fusion strains of Aspergillus niger and Aspergillus oryzae were optimized by orthogonal test. The results showed that the optimum conditions are rotational rate 180 r/min, fermentation temperature 27 ℃, inoculation amount 12%, medium volume 20 ml/250-ml shake flask and fermentation period 7 days. By these, the activity of β -glucosidase reaches 5.312 U/ml.