3,5-二硝基水杨酸比色法测定还原糖含量的研究

Study on Determination of Reducing Sugar Content Using 3,5-Dinitrosalicylic Acid Method

比较了两种DNS试剂测定还原糖含量的影响因素。探讨了试剂添加量、显色时间、测定波长以及显色后存放时间对测量结果的影响。结果表明,DNS试剂的用量1.5ml,沸水浴显色时间5min,显色定容30min后,分别在540nm和550nm波长条件下测定,数据的准确性和稳定性较好。

Two kinds of 3, 5-dinitrosalicylic acid （DNS） agents were respectively used for determination of reducing sugar content, and effects on determination results by the factors, such as amount of DNS reagent developing time, wavelength and storage time were discussed. The results showed that on the conditions of reagent amount around 1.5 ml, boiling bath time not less than 5 minutes, and standing for 30 minutes after developing, at the wavelength of 540 nm and 550 nm for reagent one and two respectively, the accurate results can be acquired.